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Zhongguo Zhongyao Zazhi 2018-Feb

[Influence of light on gene expression of key synthesis enzyme genes FtANR and FtLAR about proanthocyanidin in seeds of homologous plant of food and medicine Fagopyrum tataricum].

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Odkaz sa uloží do schránky
Chun-Li Jiang
Xin-Yao Su
Ya-Chun Xu
Er-Yi Wu
Yu-Hua Shi
Dong Zhang
Qing-Fu Chen
Wei Sun
Jian-Ping Xue

Kľúčové slová

Abstrakt

Tartary buckwheat Fagopyrum tataricum is an important medicinal and functional herb due to its rich content of flavonoids in the seeds. F.tataricum exhibited good functions for free radicals scavenging, anti-oxidation, anti-aging activities. Although much genetic knowledge of the synthesis, regulation, accumulation of rutin, the genetic basis of proanthocyanidins(PAs) in tartary buckwheat and their related gene expression changes under different lights(blue, red, far red, ultraviolet light) remain largely unexplored. In this study, we cloned one anthocyanidin reductase gene(ANR) and two leucocyanidin reductase gene(LAR) named FtANR,FtLAR1,FtLAR3 involved in formation of(+)-catechin and(-)-epicatechin precusor proanthocyanidin by digging out F. tataricum seed transcriptome data. The expression data showed that the opposite influence of red light on these gene transcript level compared to others lights. The expression levels of FtANR and FtLAR1 decreased and FtLAR3 appeared increment after exposed in the red light, while the expression levels of those genes appeared opposite result after exposed in the blue and far red light.

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