The novel multi cross-linked enzyme aggregates of protease, lipase, and catalase production from the sunflower seeds, characterization and application.

The cross-linked enzyme aggregates (CLEAs) have numerous economic advantages in the industrial bio catalysis. In the present study, the multi CLEAs containing protease, catalase, and lipase from the sunflower seeds using starch as a cofeeder as well as bovine serum albumin (BSA) are designed and prepared successfully. After optimization, multi CLEAs of enzyme have been prepared with ammonium sulfate (55% w/v), glutaraldehyde (100 mM), and 8 mg/mL of starch or 20 mg/mL of BSA. The activity recovery of protease, catalase, and lipase multi CLEAs-starch are 87, 61, and 60%, respectively. Whereas, CLEAs prepared with BSA are 74, 61, and 50% activity and multi CLEAs only 60, 44, and 41% of protease, catalase, and lipase, respectively. The multi CLEAs were used to catalyze the reactions for enhanced washing process. After adding multi CLEAs-starch, the stain removal percentage of detergents is enhanced by 83%.The present study reports a high stability, simplicity, low cost, and recyclability of the novel multi CLEAs from the sunflower seeds that make them efficient as a highly active biocatalysts in the biotechnological applications. We believe that these novel multi CLEAs present a new approach to the synthesis of multi enzyme biocatalysts from the cheap and friendly environmental sources.