High-Throughput Screening of Free Proline Content in Rice Leaf under Cadmium Stress Using Hyperspectral Imaging with Chemometrics

Tracking of free proline (FP)-an indicative substance of heavy metal stress in rice leaf-is conducive to improve plant phenotype detection, which has important guiding significance for precise management of rice production. Hyperspectral imaging was used for high-throughput screening FP in rice leaves under cadmium (Cd) stress with five concentrations and four periods. The average spectral of rice leaves were used to show differences in optical properties. Partial least squares (PLS), least-squares support vector machine (LS-SVM) and extreme learning machine (ELM) models based on full spectra and effective wavelengths were established to detect FP content. Genetic algorithm (GA), competitive adaptive weighted sampling (CARS) and PLS weighting regression coefficient (Bw) were compared to screen the most effective wavelengths. Distribution map of the FP content in rice leaves were obtained to display the changes in the FP of leaves visually. The results illustrated that spectral differences increased with Cd stress time and FP content increased with Cd stress concentration. The best result for FP detection is the ELM model based on 27 wavelengths selected by CARS and R_p is 0.9426. Undoubtedly, hyperspectral imaging combined with chemometrics was a rapid, cost effective and non-destructive technique to excavate changes of FP in rice leaves under Cd stress.

Keywords: cadmium stress; chemometrics; free proline; hyperspectral image; phenotype; rice leaf.