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7 výsledky
Transgenic Brassica chinensis plants expressing a bacterial codA gene exhibit enhanced tolerance to extreme temperature and high salinity.
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Transgenic Brassica compestris L. spp. chinensis plants expressing a choline oxidase (codA) gene from Arthrobacter globiformis were obtained through Agrobacterium tumefaciens-mediated transformation. In the transgenic plants, codA gene expression and its product transportation to chloroplasts were
Enzymic assay for lecithin in amniotic fluid.
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We describe an enzymic method for determining lecithin in amniotic fluid. Phospholipase D from savoy cabbage is used to liberate choline from lecithin, and the liberated choline is oxidized to betaine and hydrogen peroxide by choline oxidase from Arthrobacter globiformis. The hydrogen peroxide forms
Enzymatic determination of phospholipase D activity with choline oxidase.
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A new enzymatic method was developed for the assay of phospholipase D [phosphatidylcholine phosphatidohydrolase EC 3.1.4.4] from cabbage leaves using choline oxidase from Arthrobacter globiformis cells. The method was based on the estimation of choline by the following series of enzymatic reactions
Determination of trimethylamine in spinach, cabbage, and lettuce at alkaline pH by headspace solid-phase microextraction.
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Trimethylamine (TMA) found in some leafy vegetables, such as spinach, cabbage, and lettuce, at alkaline pH was identified and quantified using headspace solid-phase microextraction and gas chromatography-mass spectrometry (HS-SPME and GC-MS). HS-SPME conditions were optimized at an adsorption
Enhanced tolerance of transgenic Brassica juncea to choline confirms successful expression of the bacterial codA gene.
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Brassica juncea cv. Pusa Jaikisan was transformed with the codA gene for choline oxidase from Arthrobacter globiformis with an aim to introduce glycine betaine biosynthetic pathway, as it lacks any means to synthesize glycine betaine. Western blot analysis revealed the presence of choline oxidase in
Cloning, sequencing and salt induced expression of PEAMT and BADH in oilseed rape (Brassica napus).
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Agriculture productivity is severely hampered by soil salinity, drought and other environmental stresses. Studies on stress-resistant plants (halophytes, xerophytes, accumulating plants for specific toxic ions) have illuminated some mechanisms of stress tolerance in plants at metabolic or molecular
In-situ localization and biochemical analysis of bio-molecules reveals Pb-stress amelioration in Brassica juncea L. by co-application of 24-Epibrassinolide and Salicylic Acid.
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Lead (Pb) toxicity is a major environmental concern affirming the need of proper mitigation strategies. In the present work, potential of combined treatment of 24-Epibrassinolide (24-EBL) and Salicylic acid (SA) against Pb toxicity to Brassica juncea L. seedlings were evaluated. Seedlings
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