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Correlation-Based Network Analysis of Metabolite and Enzyme Profiles Reveals a Role of Citrate Biosynthesis in Modulating N and C Metabolism in Zea mays.

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To investigate the natural variability of leaf metabolism and enzymatic activity in a maize inbred population, statistical and network analyses were employed on metabolite and enzyme profiles. The test of coefficient of variation showed that sugars and amino acids displayed opposite trends in their

Plant uptake of depleted uranium from manure-amended and citrate treated soil.

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Six plant species were tested for their ability to accumulate depleted uranium in their above-ground biomass from deployed munitions contaminated soil in New Mexico. In greenhouse experiments, Kochia (Kochia scoparia L. Schrad.) and pigweed (Amaranthus retroflexus L) were grown with steer manure

Citrate ester substitutes for di-2-ethylhexyl phthalate: In vivo reproductive and in vitro cytotoxicity assessments

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Di-2-ethylhexyl phthalate (DEHP) is frequently used as a plasticizer for wrapping films, in toys, and in medical devices. Previous studies demonstrated that DEHP in mouse reduced testicular and epididymis weights, suppressed levels of serum testosterone, luteinizing hormone, and follicle-stimulating

Regulation of expression of the mitochondrial and peroxisomal forms of citrate synthase in maize during germination and in response to light.

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Expression of genes encoding the mitochondrial and peroxisomal forms of citrate synthase (EC 2.3.3.1) was studied in maize (Zea mays L.) in scutella during germination and in leaves depending on light regime. During germination, citrate synthase activity increased in scutella both in mitochondria

High bioavailability iron maize (Zea mays L.) developed through molecular breeding provides more absorbable iron in vitro (Caco-2 model) and in vivo (Gallus gallus).

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BACKGROUND Iron (Fe) deficiency is the most common micronutrient deficiency worldwide. Iron biofortification is a preventative strategy that alleviates Fe deficiency by improving the amount of absorbable Fe in crops. In the present study, we used an in vitro digestion/Caco 2 cell culture model as

Effects of oral exposure to the phthalate substitute acetyl tributyl citrate on female reproduction in mice.

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Acetyl tributyl citrate (ATBC), is a phthalate substitute used in food and medical plastics, cosmetics and toys. Although systemically safe up to 1000 mg kg-1 day-1 , its ability to cause reproductive toxicity in females at levels below 50 mg kg-1 day-1 has not been examined. This study evaluated

Effect of anion channel antagonists and La3+ on citrate release, Al content and Al resistance in maize roots.

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The correlation between organic acid anion release and Al content was examined in two maize (Zea mays L.) inbred lines, Cat 100-6 (Al-resistant) and S 1587-17 (Al-sensitive) treated with anion channel antagonists and La3+, a cation channel blocker. In the intact roots of Al-resistant maize, the

Transcriptional regulation of fatty acid synthase gene and ATP citrate-lyase gene by Sp1 and Sp3 in rat hepatocytes(1).

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When two copies of the sequences spanning -57 to -35 of the fatty acid synthase (FAS) or -64 to -41 of the ATP citrate-lyase (ACL) gene linked to a reporter gene were transfected into primary cultured hepatocytes, the reporter activities significantly increased in response to insulin/glucose

Purification and characterization of the reconstitutively active citrate carrier from maize mitochondria.

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The citrate carrier from maize (Zea mays) shoot mitochondria was solubilized with Triton X-100 and purified by sequential chromatography on hydroxyapatite and hydroxyapatite/celite in the presence of cardiolipin. SDS-gel electrophoresis of the purified fraction showed a single polypeptide band with

Metabolism of trans-Aconitic Acid in Maize : I. PURIFICATION OF TWO MOLECULAR FORMS OF CITRATE DEHYDRASE.

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Trans-aconitate synthesis via citrate dehydrase was determined in crude extracts of maize (Zea mays L.) coleoptiles. Two molecular forms of this enzyme were purified by substrate-specific elution from DEAE-cellulose, ammonium sulfate precipitation, and gel filtration. Each molecular form migrates as

Citrate synthetase in mitochondria and glyoxysomes of maize scutellum.

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Mitochondria and glyoxysomes were isolated from scutella of maize (Zea mays L.) by density gradient centrifugation. Citrate synthetase was partly solubilized from the organelles by sonication. The sonicated organelle suspensions were centrifuged at high speed, and the supernatants were used as

Rapid-growth responses of corn root segments: Effect of citrate-phosphate buffer on elongation.

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The effects of citrate-phosphate buffer on the elongation rate of 2 mm root segments of Zea mays L. was investigated under O2 and N2 conditions by comparing with the effects in non-buffered solution. The buffer at pH 7.0 has an inhibiting effect on the elongation rate of root segments. At pH 4.0 the

Metabolism of trans-Aconitic Acid in Maize : II. Regulatory Properties of Two Compartmented Forms of Citrate Dehydrase.

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Kinetics of two molecular forms of K-dependent citrate dehydrase in maize (Zea mays L.) are reported. The isozymes, designated CD I and CD II, were found to be compartmented in mitochondria and cytosol, respectively.CD I exhibited hyperbolic kinetics with respect to both citrate and potassium with

Chelant-assisted phytoextraction and accumulation of Zn by Zea mays.

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Zea mays plants were exposed to soils with concentrations of Zn ranging from 64 to 1800 mg kg(-1) dw, and the efficiency of three selected chelating agents (trisodium citrate (CI), disodium oxalate (OX) and disodium dihydrogen ethylene-diamine-tetraacetate (EDTA)) in enhancing metal phytoextraction

Regulation of mouse hepatic ATP-citrate lyase activity by dietary fat. Evidence for the presence of inactive enzyme.

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The induction of ATP-citrate lyase activity in mouse liver dietary carbohydrate (glucose) in markedly reduced by including in the diet a source of polyunsaturated fatty acids. Within 72 h after changing from a standard mouse chow diet to a high carbohydrate diet containing 15% (W/W) of hydrogenated

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