Disclosed is a novel method for the production of a substance which accelerates the hatching of potato cyst nematode eggs using root cells of plants of Solanaceae family.In this method, the cultivation of the root cells may be carried out in a specific medium, or, alternatively, the cells obtained by transformation of the root cells with Ri plasmid T-DNA is used.The disclosed method can be applied for the prevention of the damage by the cyst nematodes in potato cultivation.

Harima Chemicals, Inc.

Method of producing potato cyst nematode hatching stimulus

Plant CLAVATA3/ESR-related (CLE) peptides have diverse roles in plant growth and development. We have isolated and characterized the function of five new CLE genes from the potato cyst nematode Globodera rostochiensis. Unlike typical plant CLEs that contain a single CLE motif, four of the five Gr-CLE genes encode CLE proteins with multiple CLE motifs. These Gr-CLEs were found to be specifically expressed within the dorsal esophageal gland cell of nematode parasitic stages, suggesting a role for their encoded proteins in plant parasitism. Overexpression of Gr-CLEs in Arabidopsis mimicked overexpression of plant CLEs and Gr-CLE proteins could rescue the Arabidopsis clv3-2 mutant phenotype when expressed within meristems. A short root phenotype was observed when synthetic GrCLE peptides were exogenously applied to roots of Arabidopsis or potato similar to the overexpression of Gr-CLEs in Arabidopsis and potato hairy roots. These results reveal that G. rostochiensis CLEs with either single or multiple CLE motifs function similarly to plant CLEs and that CLE signaling components are conserved in both Arabidopsis and potato roots. Transgenic potato hairy roots expressing Gr-CLE-1 or Gr-CLE-4 dsRNA were generated. There was an approximately 50% reduction in the average number of cysts per root in the Gr-CLE-1 or Gr-CLE-4 dsRNA transgenic lines when compared with the infected control lines, indicating that silencing nematode CLE genes through host-derived RNAi may generate novel resistance against potato cyst nematodes in transgenic potatoes.

Wang; Xiaohong

Lu; Shunwen

The United States of America, as represented by the Secretary of Agriculture

Generating transgenic potatoes with novel resistance to potato cyst nematodes by silencing nematode parasitism genes of CLE -1 and CLE-4s

The nucleotide sequence of a 992 bp region of cDNA and the nucleotide sequence of a 1973 bp (or a 1913 bp) of genomic DNA of the Gr-cm-1 gene were determined for G. rostochiensis. PCR primers and probes specific for G. rostochiensis and G. pallida were generated. PCR assays, including a real-time TaqMan PCR were used to identify G. rostochiensis and G. pallida and to differentiate G. rostochiensis from G. pallida. Transgenic hairy roots expressing Gr-cm-1 dsRNA were generated. There was a 52% reduction in the average number of females per root in the Gr-cm-1 dsRNA transgenic lines when compared with the infected control lines.

cysts/ľuľok zemiakový

Method of producing potato cyst nematode hatching stimulus

Generating transgenic potatoes with novel resistance to potato cyst nematodes by silencing nematode parasitism genes of CLE -1 and CLE-4s

Chorismate mutase gene from the potato cyst nematode Globodera rostochiensis

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