dental plaque/phosphatase

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Ultrastructural localization of alkaline and acid phosphatase activities in dental plaque.

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Ultrastructural cytohistochemical techniques showed presence of acid and alkaline phosphatases in dental plaque. Both phosphatases had intra- and extramicrobial localization. In the extracellular matrix, phosphatases were associated with small vesicles of bacterial origin, or were freely scattered

[Ultrastructural cytochemistry of acid phosphatase and pyrophosphatase in human dental plaque].

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Some immunohistochemical experiments aiming at the electron-microscopic in situ identification of a dental plaque microorganism--Streptococcus mutans.

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The aim of the present investigation was to test a procedure useful for the electron-microscopic in situ identification of the presumptive cariogenic microorganism Streptococcus mutans (serotype d) growing in the human dental plaque. For this purpose, different parameters of an indirect

Hydrolysis of triclosan monophosphate by dental plaque and selected species of oral micro-organisms.

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Triclosan monophosphate is a phosphorylated derivative of the antimicrobial agent, triclosan. In comparison with triclosan, it is highly soluble in aqueous solutions. It is hypothesized that, within the oral environment, triclosan monophosphate (which may be devoid of antimicrobial activity) will be

Release of lysosomal hydrolases from bone explants affected by dental plaque.

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Lysosomal hydrolases as indicators of plaque-induced bone resorption in tissue culture were studied. Fetal rat bones cultured in a synthetic medium containing sonicated and filtrated human dental plaque were used as bones to be resorbed. Acid phosphatase and beta-glucuronidase were found to be the

The effect of pH, temperature and plaque thickness on the hydrolysis of monofluorophosphate in experimental dental plaque.

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Monofluorophosphate (MFP), an anti-caries agent commonly used in toothpaste, is known to be degraded to fluoride and orthophosphate by bacterial phosphatases in dental plaque. We have examined the effect of pH, temperature, plaque thickness and some ions on this process. Both natural plaque and

The effect of dental plaque grown in the presence of xylitol or sucrose on bone resorption in vitro.

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Bone culture was used as an experimental model in studying the ability of dental plaque grown in the presence of xylitol or sucrose to induce bone resorption. Plaque samples were collected in young adults after six or ten days with no oral hygiene and with frequent use of xylitol- or

Slot immunoblot assay for detection and quantitation of periodontal disease-associated microorganisms in dental plaque.

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A rapid method for qualitative and quantitative detection of specific oral microorganisms from subgingival dental plaque is described. Plaque samples were suspended in phosphate-buffered saline containing protease inhibitors and 0.5% formaldehyde, briefly sonicated to disperse bacterial aggregates,

Comparison of three different methods for evaluation of Helicobacter pylori (H.P.) in human dental plaque.

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Helicobacter pylori (H.p.) is a microorganism involved in peptic ulcer disease. To clarify the role of human dental plaque as a reservoir of H.p. and to compare different methods of investigation the authors studied 20 patients, 17 males main age 56 +/- 12 and 3 females 52 +/- 7, gastro-duodenal

An enzyme histochemical study of dental plaque and calculus.

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The following investigation was undertaken to study the location in the dental plaque and calculus of certain enzyme activities and to compare the patterns obtained with those of the normal hard tissue formation. Supragingival and subgingival calculus attached to the root surfaces of 30 extracted

Biotyping, serotyping and phage typing of Streptococcus faecalis isolated from dental plaque in the human mouth.

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Thirty Streptococcus faecalis isolates from mixed dental plaque samples were classified into four groups on the basis of biotype, tetracycline susceptibility, phage type and serotype combinations. The organisms were from patients on haemodialysis, from staff of the dialysis unit, and from controls.

Alkaline phosphatase as a periodontal disease marker.

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BACKGROUND The potential of alkaline phosphatase (ALP) as an important diagnostic marker of gingival crevicular fluid (GCF) has been the subject to investigation since 1970. ALP is stored in specific granules and secretory vesicles of the neutrophils and is mainly released during their migration to

In vitro hydrolysis of monofluorophosphate by dental plaque microorganisms.

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Enzymic hydrolysis of sodium monofluorophosphate by suspensions of dental microorganisms has been demonstrated at pH 5.1, pH 7.0, and pH 8.4, using a fluoride-selective electrode. The extracellular medium from viable Streptococcus mutans K1R cells contained low MFPase and paranitrophenyl phosphatase

Simultaneous hybridization and subsequent colour detection of subgingival bacterial DNA on colony lifts.

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This paper reports the development of a protocol allowing hybridization and detection of DNA fixed to nylon colony lifts from up to three species of bacteria simultaneously. Half ml samples of serial dilutions of pure cooked-meat broth (CMB) cultures of Capnocytophaga ochracea, Actinobacillus

Clinical Effects of Stabilized Stannous Fluoride Dentifrice in Reducing Plaque Microbial Virulence I: Microbiological and Receptor Cell Findings.

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OBJECTIVE Lipopolysaccharides (LPSs) and lipoteichoic acids (LTAs), or bacterial endotoxins, bind with Toll-like receptors (TLRs) that are expressed on host cells of the periodontium, thereby contributing to the periodontal pathogenicity of oral bacteria. Stannous fluoride (SnF2), an antibacterial

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