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13 výsledky
Absence of sterol-specific complexes at active zones of degenerating and regenerating frog neuromuscular junctions.
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Freeze-fracture combined with filipin treatment has been used as a cytochemical probe for membrane cholesterol. As previously shown at the frog neuromuscular junction, distinctive sterol-specific complexes were formed on the presynaptic membrane after filipin treatment, except at active zones. The
Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein.
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Cytoplasmic assembly of Sm-class small nuclear ribonucleoproteins (snRNPs) is a central process in eukaryotic gene expression. A large macromolecular complex containing the survival of motor neurons (SMN) protein is required for proper snRNP assembly in vivo. Defects in SMN function lead to a human
Decreased polysomal HSP-70 may slow polypeptide elongation during skeletal muscle atrophy.
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Slowed elongation rate is the apparent cause of the rapid decrease in rat soleus muscle protein synthesis rate during non-weight bearing. We found that elongation factor 2 was not phosphorylated and thus could not explain the slowed elongation rate. However, we observed a 44 +/- 19 and 28 +/- 14%
Decay of murine intestinal epithelial cells under extrusion.
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Electron microscopic findings of this study revealed 2 types of extrusion of brush border cells from the summits of intestinal villi: a) By expansion of initial vacuolation the degenerating cell enlarges and elevates itself above the level of the extrusion zone. Cells about to lift become sphered
Effect of moderate ethanol intake on the heart: biochemical and morphological studies with isolated cardiomyocytes from rats fed a low-protein diet.
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OBJECTIVE
It has been shown that chronic consumption of ethanol can impair myocardial function and result in morphological changes of the heart. The purpose of this work was to evaluate relations between changes in energy metabolism and changes of cell morphology.
METHODS
Long-term effects of
3-Hydroxyglutaric acid moderately impairs energy metabolism in brain of young rats.
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3-Hydroxyglutaric acid (3HGA) accumulates in the inherited neurometabolic disorder known as glutaryl-CoA dehydrogenase deficiency. The disease is clinically characterized by severe neurological symptoms, frontotemporal atrophy and striatum degeneration. Because of the pathophysiology of the brain
New approach to reduce allograft tissue immunogenicity. Experimental data.
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OBJECTIVE
Rejection is thought to contribute to the degeneration of valved allografts. Most proposed methods of decellularisation allow usage of treated valves in pulmonic position. We developed a new protocol of devitalization, which provides cell death and suppression of calcification using
[Investigations on the glycocalyx with toluidin blue staining (author's transl)].
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The study was concerned with the molecular structure of the erythrocyte membrane and its anisotropic staining with toluidine blue. Enzymatic or chemical degradation impairs the topo-optical staining. Decreased anisotropy results also from digitonin treatment of glutaraldehyde fixed cells. Incubation
Retinal stimulates ATP hydrolysis by purified and reconstituted ABCR, the photoreceptor-specific ATP-binding cassette transporter responsible for Stargardt disease.
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Many substrates for P-glycoprotein, an ABC transporter that mediates multidrug resistance in mammalian cells, have been shown to stimulate its ATPase activity in vitro. In the present study, we used this property as a criterion to search for natural and artificial substrates and/or allosteric
Identification of nuclear import mechanisms for the neuronal Cdk5 activator.
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The activation of Cdk5 by p35 plays a pivotal role in a multitude of nervous system activities ranging from neuronal differentiation to degeneration. A fraction of Cdk5 and p35 localizes in the nucleus where Cdk5-p35 exerts its functions via protein phosphorylation, and p35 displays a dynamic
Vesicular monoamine transporter 2 regulates the sensitivity of rat dopaminergic neurons to disturbed cytosolic dopamine levels.
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An abnormal accumulation of cytosolic dopamine resulting in reactive oxygen species and dopamine-quinone products may play an important role in the rather selective degeneration of substantia nigra pars compacta (SNc) dopaminergic neurons in Parkinson's disease. The neuronal-specific vesicular
RCS rat retinal rod outer segment membranes exhibit different cholesterol distributions than those of normal rats.
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Royal College of Surgeons (RCS) rats exhibit an hereditary defect in phagocytosis of the tips of the photoreceptor cell rod outer segments (ROS) which leads to degeneration of the retinal visual cells. The lipid composition of outer segment membranes of these rats was analysed and compared to those
Caspases indirectly regulate cleavage of the mitochondrial fusion GTPase OPA1 in neurons undergoing apoptosis.
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The critical processes of mitochondrial fission and fusion are regulated by members of the dynamin family of GTPases. Imbalances in mitochondrial fission and fusion contribute to neuronal cell death. For example, increased fission mediated by the dynamin-related GTPase, Drp1, or decreased fusion
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