[Tumor necrosis factor-alpha enhances type I inositol 1, 4, 5-triphosphate receptor expression in rat glomerular afferent arterioles smooth muscle cells].
Ključne besede
Povzetek
OBJECTIVE
To study the effect of tumor necrosis factor-alpha (TNF-alpha) on type I inositol 1, 4, 5-triphosphate receptor (IP(3) R) expression in rat glomerular afferent arterioles smooth muscle cells (RASMC).
METHODS
Isolation and culture of RASMC and detection of type I IP(3) R protein and type I IP(3) R mRNA in RASMC after TNF-alpha treatment were carried out with Western blot and Northern blot assay.
RESULTS
TNF-alpha enhanced the expression of type I IP(3) R protein and type I IP(3) R mRNA in RASMC; TNF-alpha did not influence the half life of type I IP(3) R mRNA in RASMC treated with TNF-alpha.
CONCLUSIONS
TNF-alpha plays a role in the development of renal dysfunction. The mechanisms may be that TNF-alpha takes part in the change of signal transduction in RASMC. TNF-alpha may act on the promoter of type I IP(3) R mRNA in RASMC and results in the expression of type I IP(3) R protein that stimulates release of intracellular Ca(2+) in RASMC and induces contraction of RASMC. The renal blood flow diminution leads to the development of renal dysfunction.
