Antioxidant activity of eugenol in Piper betel leaf extract.

Extracts of Piper betel are used for the treatment of various ailments since ages due to its essential properties like antioxidant, anticancer, anti-allergic etc. In the present study antioxidant activity for Piper betel leaf extract and Eugenol was assessed. Eugenol was taken as marker compound.Nitric oxide, Hydroxyl radical and Reducing power assay methods were carried out for assessment of antioxidant activity of Piper betel.The antioxidant activity for Nitric oxide, Hydroxyl radical and Reducing power assay at 1000 to 62.5μg/ml was performed. The antioxidant activity of Piper betel leaf extract exhibited the IC50 value for Nitric oxide and Hydroxyl radical >1000 whereas Eugenol exhibited the IC50 value 114.34± 0.46 and 306.44 ± 5.28 respectively, for reducing power assay (RPA) Piper betel leaf extract and Eugenol revealed the RPA value ranging from 0.44-0.08 and 0.53-0.12.The benefits of Piper betel have been mentioned in our ancient texts. Keeping in view the emergence of various diseases and the benefits of Piper betei, there is need that every effort should be made to revive this treasure of nature into our daily supplement.