Genetic analysis and Immunoelectron Microscopy of Wild and Mutant Strains of the Rubber Tree Endophytic Bacterium Serratia Marcescens Strain ITBB B5-1 Reveals Key Roles of A Macrovesicle in Storage and Secretion of Prodigiosin.
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Povzetek
Rubber tree is an economically important tropical crop. Its endophytic bacterial strain Serratia marcescens ITBB B5-1 contains intracellular a macrovesicles and red pigment. In this research, the red pigment was identified as prodigiosin by quadrupole time-of-flight mass spectrometry. Prodigiosin has a wide range of potential medical values such as anti-cancer and anti-organ transplant rejection. The strain ITBB B5-1 accumulated prodigiosin up to 2000 mg/L, which is higher production compared to most known Serratia strains. The formation of the macrovesicle and prodigiosin biosynthesis were highly associated and were both temporal and temperature dependent. A mutant strain B5-1mu that failed to produce prodigiosin was obtained by ultraviolet mutagenesis. Whole genome sequencing of wild-type and mutant strains indicated that the PigC gene encoding the last-step enzyme in the prodigiosin biosynthesis pathway was mutated in B5-1mu by a 17-bp deletion. TEM analysis showed that the macrovesicle was absent in the mutant strain, indicating that formation of the macrovesicle relied on prodigiosin biosynthesis. Immunoelectron microscopy using prodigiosin-specific antiserum showed the presence of prodigiosin in the macrovesicle, the cell wall, and the extracellular vesicles, while immuno-reaction was not observed in the mutant cell. These results indicate that the macrovesicle serves as a storage organelle of prodigiosin, and secrete prodigiosin into cell envelop and culture medium as extracellular vesicles.