alpha galactosidase/oryza sativa

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Expression of rice (Oryza sativa L. var. Nipponbare) alpha-galactosidase genes in Escherichia coli and characterization.

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Prijava / prijava

Two putative alpha-galactosidase genes from rice (Oryza sativa L. var. Nipponbare) belonging to glycoside hydrolase family 27 were cloned and expressed in Escherichia coli. These enzymes showed alpha-galactosidase activity and were purified by Ni Sepharose column chromatography. Two purified

alpha-Galactosidase from cultured rice (Oryza sativa L. var. Nipponbare) cells.

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Prijava / prijava

The alpha-galactosidase from rice cell suspension cultures was purified to homogeneity by different techniques including affinity chromatography using N-epsilon-aminocaproyl-alpha-D-galactopyranosylamine as the ligand. From 11 l of culture filtrate, 28.7 mg of purified enzyme was obtained with an

Crystallization and preliminary X-ray crystallographic studies of rice alpha-galactosidase.

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Prijava / prijava

alpha-Galactosidases catalyze the hydrolysis of galactooligosaccharides and galactopolysaccharides to alpha-galactose residues and are widely distributed in microorganisms, plants and animals. alpha-Galactosidase from rice (Oryza sativa L. ssp. japonica) was crystallized by the hanging-drop

Alkaline alpha-galactosidase degrades thylakoid membranes in the chloroplast during leaf senescence in rice.

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Here, we studied the functional role of a chloroplast alkaline alpha-galactosidase (OsAkalphaGal) in the breakdown of thylakoid membranes during rice (Oryza sativa) leaf senescence. We assayed the enzyme activity of recombinant OsAkalphaGal with different natural substrates and examined the effect

Functional analysis of the structural domain of ARF proteins in rice (Oryza sativa L.).

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Auxin response factors (ARFs) are key regulators of plant growth and development. Through interaction with auxin/indole acetic acid (Aux/IAA) proteins, they influence the expression of auxin response genes. An ARF gene family has been predicted in rice, but the functions of the individual structural

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