Placental Tissue samples collection: • Placental tissue samples (surgically obtained during the caesarean section from 50 pregnant women) will be selected: - 25 PE patients undergoing the caesarean section following confirmation of diagnosis clinically by blood pressure measurement and by urine analysis to detect the proteinuria. - 25 control women undergoing the caesarean section. Blood samples. Urinary samples.

Pre-Eclampsia

Role and Expression of MXRA5 in Placental Connective Tissue Remodelling in Preeclampsia

Role and Expression of Matrix-remodelling Associated 5 Protein (MXRA5) in Connective Tissue Remodelling of Placenta in Preeclampsia

Shimaa Ahmed Abdelrahman

Principal Investigator

Assiut University

Patients with and without colorectal cancer

Colorectal Cancer

All patients included in the study will have blood sample

Blood sample

Chu Reims

Identification of New Diagnostic Protein Markers for Colorectal Cancer

Identification of New Diagnostic Protein Markers for Colorectal Cancer in Circulating Tumor Exosomes

Sensitivity and specificity of macromolecules

Sensitivity and specificity of metallo proteases

CHU de Reims

Colon Cancer

Adenocarcinoma

LRP1 and Colon Cancer

Prognostic Impact of Immunohistochemical and Molecular Expression of the Endocytosis Receptor LRP1 in Colonic Adenocarcinomas

Blood samples from healthy volunteers will be obtained at one time point.Peripheral blood samples will be obtained into tubes with no additive (BD Vacutainer System, Plymouth, UK).Samples will be processed to serum. Serum concentrations of sFas will be quantitative determinated by a sandwich enzyme immunoassay technique (ELISA)using specific anti-Fas MoAbs, Human sFas Immunoassay. Serum concentrations of sFasL will be quantitative determinated by a sandwich enzyme immunoassay technique (ELISA) using specific anti-Fasl MoAbs, Human sFas Immunoassay. Serum concentration of IL - 17 will be quantitative determinated by a sandwich enzyme immunoassay technique (ELISA) using Human IL-17 Immunoassay. . Peripheral blood samples for measurement of oxidative burst in neutrophils will be collected into heparinised blood tube. burst neutrophil production will be determined quantitatively by flow cytometry as described by Rothe using a commercial kit Bursttest Kit.

Colonic Neoplasms

Patients with colorectal cancer undergo open laparotomy and colorectal resection

Therapeutic conventional colorectal surgery

Patients with colorectal cancer undergo laparoscopic colorectal resection

Therapeutic laparoscopic colorectal surgery

Informed consent will be obtained.Blood samples will be obtained at one time point. .Samples will be processed to serum, using a refrigerated centrifuge, then stored at -80C until analysis. Peripheral blood samples for measurement of oxidative burst in neutrophils will be collected into heparinised blood tube.Serum concentrations of sFas will be quantitative determinated by a sandwich enzyme immunoassay technique (ELISA) using specific anti-Fas MoAbs, Human sFas Immunoassay (Code: DFS00; QUANTIKINE R&D Systems Inc, Minneapolis, USA). Serum concentrations of sFasL will be quantitative determinated by a sandwich enzyme immunoassay technique (ELISA) using specific anti-Fasl MoAbs, Human sFas Immunoassay (Code: DFS00; QUANTIKINE R&D Systems Inc, Minneapolis, USA).Respiratory burst neutrophil production will be determined quantitatively by flow cytometry using a commercial kit Bursttest Kit (Cat. No: 10-0200; ORPEGEN Pharma, Germany)

colorectal neoplasms/protease

Role and Expression of MXRA5 in Placental Connective Tissue Remodelling in Preeclampsia

Identification of New Diagnostic Protein Markers for Colorectal Cancer

LRP1 and Colon Cancer

Double Blinded Randomized Clinical Trial of the Effect of Open Versus Laparoscopic Colectomy on Neutrophils in Patients With Colon Cancer

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