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Stran 1 iz 59 rezultatov
In vitro formation and development of floral buds on tobacco stem explants: effects of kinetin and other factors.
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Stem segments were excised from plants of Wisconsin 38 tobacco (Nicotiana tabacum L.) in three regions differing in their distance below the inflorescence. They were cultured in vitro in 8- or 16-hr days. After 8 weeks, floral and vegetative buds were counted, and extent of floral development was
Kinetin incorporated into tobacco callus ribosomal RNA and transfer RNA preparations.
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Kinetin, N(6)-furfuryladenine, was incorporated into tobacco (nicotiana tabacum L., var. Wis. No. 38) callus RNA isolated from rapidly growing tissue cultured in the presence of N(6)-furfuryladenine-8-(14)C or unlabeled kinetin. Approximately 0.7% of the radioactivity in the labeled kinetin added to
Nucleic acid and protein synthesis in discs cut from mature leaves of Nicotiana tabacum L. and cultured on nutrient agar with and without kinetin.
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The effect of kinetin on aspects of the metabolism of discs cut from mature leaves of Nicotiana tabacum and cultured in the light on agar containing mineral salts and sucrose was studied. In the first few days of culture there was a rapid decline in chlorophyll content. Discs treated with kinetin in
A transient increase of phenylalanine ammonia-lyase transcript in kinetin-treated tobacco callus.
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In tobacco cell culture (Nicotiana tabacum L. "Bright Yellow" T-13), phenylalanine ammonia-lyase (PAL) activity was induced in response to an exogenously added kinetin. RNA blot hybridization analysis showed that a single species of PAL transcript 2.9-kb in size was detected using the PAL cDNA
Kinetin-induced Changes in delta-Aminolevulinic Acid Dehydratase of Tobacco Callus.
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Kinetin-induced changes in dry weight, soluble protein content, delta-aminolevulinic acid dehydratase activity, and chlorophyll content of two clones of Nicotiana tabacum L. callus were studied. Kinetin brought about a marked increase in the delta-aminolevulinic acid dehydratase activity of nongreen
Kinetin Reversal of NaCl Effects.
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Leaf discs of Nicotiana rustica L. were floated on NaCl in the presence of kinetin or abscisic acid. On the 5th day (14)CO(2) fixation, [(3)H]leucine incorporation, stomatal conductance, and chlorophyll content were determined. Kinetin either partially or completely reversed the inhibitory effects
A rapid effect of kinetin on rehydration of tobacco leaf tissue.
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Partly dehydrated tobacco leaf tissue (Nicotiana rustica), stripped of the lower epidermis, was used to study the effect of kinetin on the rate of rehydration. Depending on the rate of rehydration in untreated tissue, kinetin either increased or decreased rehydration rates. The response to kinetin
Water and salt stresses, kinetin and protein synthesis in tobacco leaves.
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The capacity of tobacco (Nicotiana rustica) leaf discs to incorporate l-leucine (14)C into proteins was measured. Leaf discs were obtained from plants which experienced soil water depletion, or which were exposed to a saline or osmotic stress in the root medium. The stresses were brief of relatively
DNA synthesis in cytokinin-autotrophic tobacco cells : Effect of bromodeoxyuridine, fluorodeoxyuridine, and kinetin.
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DNA isolated from various Nicotiana tabacum cell types, differing in their degree of hormone autotrophy and incubated in the presence of bromodeoxyuridine (BrdUrd), was analyzed by isopycnic CsCl gradient centrifugation. All cell types incorporate BrdUrd into DNA in such a way that hybrid DNA is
Effects of sucrose and kinetin on growth and chlorophyll synthesis in tobacco tissue cultures.
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Investigations were carried out on the effects of various combinations of sucrose and kinetin concentrations on growth and chlorophyll production in a green and a nongreen clone of pith callus of Nicotiana tabacum L. It was found that 2 milligrams per liter or higher amounts of kinetin induced
THE INFLUENCE OF KINETIN ON TOBACCO MOSAIC VIRUS PRODUCTION IN NICOTIANA GLUTINOSA LEAF DISKS.
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[Studies on the effect of kinetin on callus and tumors in organ cultures of Nicotiana glauca, N. langsdorffii and their amphidiploid hybrid].
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Substituted nitroguanidines provide cytokinin activity during in vitro cultivation of plant tissues.
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Synthetic nitroguanidine derivatives can be used as alternatives to the traditional adenine-containing cytokinins used in plant tissue culture. First, nitroguanidine derivatives (NG) mimicked the typical activity of two standard cytokinins, 6-benzylaminopurine (BAP) and 2-isopentenyladenine (2iP) in
Effect of Plant Growth Regulators on Calcium-stimulated Serine Transport into Tobacco Cells.
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The transport of serine into tobacco cells (Nicotiana tabacum L.) cultured in liquid medium was examined. Transport was inhibited approximately 50% by 2,4-dichlorophenoxyacetic acid, indoleacetic acid, alpha-naphthalene acetic acid, and kinetin at a concentration of 10 micrograms per milliliter.
Evidence for an abundant 33,000-dalton polypeptide regulated by cytokinins in cultured tobacco tissues.
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When cloned pith and leaf tissues of Nicotiana tabacum L. cv. Havana 425 are subcultured for 3 d on auxin-containing medium and labelled for 18 h with [(35)S]methionine, up to 10% of the labelled, soluble-protein fraction is found in a single band with an apparent molecular weight of approx.
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