Since PBMCs contain the full complement of epigenetic enzymes found in most tissues, the investigators evaluated the role of DNA methylation in the regulation of ES gene transcription in all the enrolled subjects. DNA was extracted from whole blood using QIAmp DNA Blood Mini Kit and its concentration was determined by NanoDrop quantification. Arrays preparation and data analysis were performed by Genomix4Life srl. High-quality DNA (500 ng) was bisulfite converted using EZ DNA methylation kit. Bisulfite converted DNA (200ng) was used for analysis of whole-genome methylation, using the HumanMethylation 450 K BeadChip, which contains 485 577 probes covering 21 231 (99%) RefSeq genes.

Chronic Migraine

Ircss Mondino Foundation

Gene Expression of Endocannabinoid System in Episodic and Chronic Migraine Patients

Peripheral Gene Expression of Endocannabinoid System Components in Episodic and Chronic Migraine Patients: a Pilot Study

CB1 protein expression in the peripheral blood mononuclear cells (PBMCs)

CB2 protein expression in the peripheral blood mononuclear cells (PBMCs)

Gene expression of the endocannabinoid receptors in the peripheral blood mononuclear cells ( PBMCs)

Gene expression of the FAAH in the peripheral blood mononuclear cells ( PBMCs)

Gene expression of the NAPE-PLD in the peripheral blood mononuclear cells ( PBMCs)

Gene expression of the DAGL in the peripheral blood mononuclear cells ( PBMCs)

Gene expression of the MAGL in the peripheral blood mononuclear cells ( PBMCs)

DNA methylation in the regulation of ES gene transcription

IRCCS National Neurological Institute "C. Mondino" Foundation

Ministry of Health, Italy

Enteral nutrition: Protein 1.5 gm/kg/day, energy (kcal) 40/kg/day, carbohydrate 67-80%, Fat 20-33%.
Oral prednisolone 40mg/day × 4 weeks and tapered to <40mg/day for next 4 weeks. If Lilli score > 0.45 after 7 days, then GC would be stopped and patients will be counselled for liver transplantation.
Oral Bovine colostrum (200 ml (20 gram) TDS × 2 months.

Severe Alcoholic Hepatitis in 'Extremis'- Defined by mDF>54

Oral Bovine colostrum 200 ml (20 gram) TDS × 2 months

Bovine colostrum

Enteral nutrition: Protein 1.5 gm/kg/day, energy (kcal) 40/kg/day, carbohydrate 67-80%, Fat 20-33%.

Enteral Nutrition

Oral prednisolone 40mg/day × 4 weeks and tapered to <40mg/day for next 4 weeks. (If Lilli score > 0.45 after 7 days, then prednisolone would be stopped)

lipase/glavobol

Gene Expression of Endocannabinoid System in Episodic and Chronic Migraine Patients

Efficacy of Combination Therapy of Glucocorticoids and Bovine Colostrum in Treatment of Severe Alcoholic Hepatitis.

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