nitrate/arabidopsis thaliana

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Nitrate acts at the Arabidopsis thaliana shoot apical meristem to regulate flowering time.

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Optimal timing of flowering, a major determinant for crop productivity, is controlled by environmental and endogenous cues. Nutrients are known to modify flowering time; however, our understanding of how nutrients interact with the known pathways, especially at the shoot apical meristem (SAM), is

Expression analysis of a high-affinity nitrate transporter isolated from Arabidopsis thaliana by differential display.

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We used the differential display technique on total RNAs from roots of Arabidopsis thaliana (L.) Heynh. plants which had or had not been induced for 2 h by nitrate. One isolated cDNA clone, designated Nrt2:1At, was found to code for a putative high-affinity nitrate transporter. Two genomic sequences

Plant Defense Stimulator Mediated Defense Activation Is Affected by Nitrate Fertilization and Developmental Stage in Arabidopsis thaliana

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Plant defense stimulators, used in crop protection, are an attractive option to reduce the use of conventional crop protection products and optimize biocontrol strategies. These products are able to activate plant defenses and thus limit infection by pathogens. However, the effectiveness of these

Isoform-Specific NO Synthesis by Arabidopsis thaliana Nitrate Reductase.

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Nitrate reductase (NR) is important for higher land plants, as it catalyzes the rate-limiting step in the nitrate assimilation pathway, the two-electron reduction of nitrate to nitrite. Furthermore, it is considered to be a major enzymatic source of the important signaling molecule nitric oxide

Dual Effect of Light on the Gibberellin- and Nitrate-Stimulated Seed Germination of Sisymbrium officinale and Arabidopsis thaliana.

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Red light (R) has a dual effect on the seed germination of the two related species Arabidopsis thaliana and Sisymbrium officinale. The two species provide different means to separate the light-effects. In S. officinale, stimulation of germination by R depends on the stimultaneous presence of nitrate

Constitutive non-inducible expression of the Arabidopsis thaliana Nia 2 gene in two nitrate reductase mutants of Nicotiana plumbaginifolia.

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We have isolated a haploid cell line of N. plumbaginifolia, hNP 588, that is constitutive and not inducible for nitrate reductase. Nitrate reductase mutants were isolated from hNP 588 protoplasts upon UV irradiation. Two of these nitrate reductase-deficient cell lines, nia 3 and nia 25, neither of

Differential expression of genes for response regulators in response to cytokinins and nitrate in Arabidopsis thaliana.

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In Arabidopsis thaliana, a number of response regulators are presumably involved in His-Asp phosphorelay signal transduction in response to environmental stimuli, such as phytohormones. Previously, it was shown that expression of a certain set of genes for response regulators are cytokinin- and

Isolation and characterization of nitrate reductase-deficient mutants of Arabidopsis thaliana.

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Chlorate resistant mutants of Arabidopsis thaliana were isolated, of which 10 exhibited a lowered nitrate reductase activity and 51 were chlorate-resistant because of an impaired uptake of chlorate. The 51 mutants of this type are all affected in the same gene. The mutants with a lowered nitrate

Expression pattern of genes encoding nitrate and ammonium assimilating enzymes in Arabidopsis thaliana exposed to short term NaCl stress.

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Key steps in nitrate nutrition and assimilation were assessed over two weeks in control and 100mM NaCl-exposed Arabidopsis thaliana (Columbia) plants. The data showed that NaCl stress lowered nitrate contents in both leaves and roots. While NaCl stress decreased ammonium contents in leaves, it

Mediated electrochemistry of nitrate reductase from Arabidopsis thaliana.

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Herein we report the mediated electrocatalytic voltammetry of the plant molybdoenzyme nitrate reductase (NR) from Arabidopsis thaliana using the established truncated molybdenum-heme fragment at a glassy carbon (GC) electrode. Methyl viologen (MV), benzyl viologen (BV), and anthraquinone-2-sulfonic

A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase.

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Nitrate reductase (NR) from the plant Arabidopsis thaliana has been employed in the development of an amperometric nitrate biosensor that functions at physiological pH. The anion anthraquinone-2-sulfonate (AQ) is used as an effective artificial electron transfer partner for NR at a glassy carbon

Regulation of a putative high-affinity nitrate transporter (Nrt2;1At) in roots of Arabidopsis thaliana.

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Putative high-affinity nitrate (NO3-) transporter genes, designated Nrt2;1At and Nrt2;2At, were isolated from Arabidopsis thaliana by RT-PCR using degenerate primers. The genes shared 86% and 89% identity at the amino acid and nucleotide levels, respectively, while their proteins shared 30-73%

Glutaredoxin AtGRXS8 represses transcriptional and developmental responses to nitrate in Arabidopsis thaliana roots

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Glutaredoxins (GRXs) are small oxidoreductase enzymes that can reduce disulfide bonds in target proteins. The class III GRX gene family is unique to land plants, and Arabidopsis thaliana has 21 class III GRXs, which remain largely uncharacterized. About 80% of A. thaliana class III

Nitrate Induction of Primary Root Growth Requires Cytokinin Signaling in Arabidopsis thaliana.

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Nitrate can act as a potent signal to control growth and development in plants. Here we show nitrate is able to stimulate primary root growth via increased meristem activity and cytokinin signaling. Cytokinin perception and biosynthesis mutants displayed shorter roots as compared to wild type plants

Reverse mutants of the nitrate reductase-deficient mutant B 25 of Arabidopsis thaliana.

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Revertants of B25, a nitrate reductase-deficient mutant of Arabidopsis thaliana (L.) Heinh, were isolated with a high frequency. All 7 independently arisen revertants were mutations in the same suppressor gene su, which is unlinked to the originally mutated gene rgn. The mutant character shows up

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