pemphigus/phosphatase

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Alkaline phosphatase immuno-enzymatic technique in the diagnosis of pemphigus vulgaris.

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Prijava / prijava

Alkaline phosphatase was used in an immuno-enzymatic procedure to detect tissue-bound and circulating antibodies in pemphigus vulgaris. Pemphigus antibodies were revealed by a direct method using alkaline phosphatase conjugated goat anti-human IgG. Cryostat sections were incubated with the specific

[Activity of acid and alkaline phosphatase in the skin of healthy persons and in those with chronic pemphigus].

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Prijava / prijava

Differential coupling of M1 muscarinic and alpha7 nicotinic receptors to inhibition of pemphigus acantholysis.

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Prijava / prijava

The mechanisms mediating and regulating assembly and disassembly of intercellular junctions is a subject of intensive research. The IgG autoantibodies produced in patients with the immunoblistering skin disease pemphigus vulgaris (PV) can induce keratinocyte (KC) dyshesion (acantholysis) via

[Characterization of lymphocytic infiltrate cells in bullous pemphigoid and pemphigus vulgaris].

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Prijava / prijava

By means of monoclonal antibodies (mab) and alkaline phosphatase anti-alkaline-phosphatase (APAAP) technique, the distribution and characteristics of lymphocytic infiltrates were studied in biopsies from 15 patients with bullous pemphigoid (b.P.) and 5 patients with pemphigus vulgaris (P.v.). The

Further support for a role for Th2-like cytokines in blister formation of pemphigus.

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Prijava / prijava

Pemphigus vulgaris and pemphigus foliaceus are commonly known as antibody-mediated bullous diseases. However, recently a role for infiltrating cells as contributors to the pathogenesis of these diseases has been suggested. The aims of our study were to characterize the immunophenotype of the

Pemphigus vulgaris IgG and methylprednisolone exhibit reciprocal effects on keratinocytes.

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Prijava / prijava

Pemphigus vulgaris (PV) is a life-threatening autoimmune disease of skin adhesion associated with IgG autoantibodies against keratinocytes (KC). Treatment of PV with systemic corticosteroids is life-saving, but the mechanism of the therapeutic action has not been fully understood. We have developed

PTPN22 1858T is not a risk factor for North American pemphigus vulgaris.

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Prijava / prijava

Alterations in the protein tyrosine phosphatase N22 (PTPN22) gene affect the threshold for lymphocyte activation. The PTPN22 1858T polymorphism leads to uninhibited T-cell receptor cascade propagation. An elevated PTPN22 1858C/T genotype frequency has been correlated with several autoimmune

Immunocytochemical detection of autoantibody deposits in Tzanck smears from patients with oral pemphigus.

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Prijava / prijava

Eighteen patients, ten affected by pemphigus vulgaris and four affected by herpes simplex of the oral mucosa, together with four healthy patients as controls, were investigated by cytologic examination of Papanicolaou stained smears obtained by scraping the oral mucosa. In all cases additional

Hepatotoxicity and liver enzyme alteration in patients with immunobullous diseases receiving immunosuppressive therapy.

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Prijava / prijava

To avoid complications of high dose corticosteroid, pemphigus patients are usually co-treated with other immunosuppressive agents. Liver enzyme abnormality occurs commonly during treatment and occasionally causes discontinuation of drugs. To assess the rate of therapy-induced hepatotoxicity in

Mitogen-activated protein kinase kinase 1/extracellular signal-regulated kinase (MEK-1/ERK) inhibitors sensitize reduced glucocorticoid response mediated by TNFalpha in human epidermal keratinocytes (HaCaT).

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Prijava / prijava

Glucocorticoids (GCs) are essential drugs administered topically or systematically for the treatment of autoimmune skin diseases such as pemphigus. However, a certain proportion of patients does not respond well to GCs. Although studies on the relationship between cytokines and GC insensitivity in

Enzyme immunoassay of antibodies to epithelial glycoprotein: increased level of antibodies in coeliac disease.

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Prijava / prijava

From the papules of a patient with massive cutaneous hyalinosis, we earlier isolated a mannose-rich glycoprotein that, by immunohistochemical methods, was also shown to be present in epithelial cells of small intestine and normal skin. A solid-phase enzyme immunoassay of IgG and IgM antibodies to

Vitamin D and skeletal health in autoimmune bullous skin diseases: a case control study.

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Prijava / prijava

BACKGROUND The presence of hypovitaminosis D in patients with autoimmune bullous skin diseases, such as pemphigus vulgaris (PV) and bullous pemphigoid (BP), is debated. In a previous study we found an increased prevalence of vertebral fractures (VFx) and hypovitaminosis D in PV and BP patients. The

Cutaneous manifestations of gastrointestinal disease: part I.

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Prijava / prijava

Cutaneous findings are not uncommonly a concomitant finding in patients afflicted with gastrointestinal (GI) diseases. The dermatologic manifestations may precede clinically evident GI disease. Part I of this 2-part CME review focuses on dermatologic findings as they relate to hereditary and

RhoA is dispensable for skin development, but crucial for contraction and directed migration of keratinocytes.

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Prijava / prijava

RhoA is a small guanosine-5'-triphosphatase (GTPase) suggested to be essential for cytokinesis, stress fiber formation, and epithelial cell-cell contacts. In skin, loss of RhoA was suggested to underlie pemphigus skin blistering. To analyze RhoA function in vivo, we generated mice with a

Desmosomal protein regulation and clinical implications in oral mucosal tissues

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Prijava / prijava

Cell-adhesion complex within a tissue is important for its stability, structural integrity, functioning, cellular migration and morphogenesis. Disruption of desmosomal cell-adhesions complex results in epithelial conditions such as epidermolysis bullosa and bullous pemphigoid. Desmosome assembly and

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