protein-losing enteropathies/protease

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Mouse mast cell protease-1 is required for the enteropathy induced by gastrointestinal helminth infection in the mouse.

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OBJECTIVE The relationship between intestinal pathology and immune expulsion of gastrointestinal nematodes remains controversial. Immune expulsion of gastrointestinal helminth parasites is usually associated with Th2 responses, but the effector mechanisms directly responsible for parasite loss have

Pancreatic proteases and oxygen-derived free radicals in acute ischemic enteropathy.

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The specific susceptibility of the intestinal mucosa to low blood flow states is related to the "physiologic" makeup of the intestinal milieu. Pancreatic proteases appear to play a crucial role in the ischemic autodigestion of the intestinal mucosa. Moreover, trypsin can activate the conversion of

[Combination of a synthetic anticholinergic agent and various enzymes (amylases, lipases, proteases, hemicellulases and cellulases) in the treatment of enteropathies].

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Evaluation of intestinal permeability and gluten sensitivity in Soft-Coated Wheaten Terriers with familial protein-losing enteropathy, protein-losing nephropathy, or both.

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OBJECTIVE To evaluate intestinal permeability and gluten sensitivity in a family of Soft-Coated Wheaten Terriers (SCWT) affected with protein-losing enteropathy (PLE), protein-losing nephropathy (PLN), or both. METHODS 6 affected adult dogs. METHODS Intestinal biopsy specimens, urine

[Anti-inflammatory actions of proteases, bromelain, trypsin and their mixed preparation (author's transl)].

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Anti-inflammatory actions of proteases, bromelain (BR), trypsin (TR) and their mixed preparation (KT) were studied mainly in rabbits using various experimental test methods. Inhibitory action of edema formation induced by carrageenin was observed to be dose dependent with oral administrations of KT.

Enzyme-linked immunosorbent assay for canine alpha 1-protease inhibitor.

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OBJECTIVE To develop and validate an ELISA for quantifying alpha 1-protease inhibitor (alpha 1-PI) in serum and fecal extracts. METHODS Affinity-purified rabbit origin canine alpha 1-PI antibodies were biotinylated and, after addition of streptavidin-horseradish peroxidase, were used as the labeled

Matriptase drives early-onset intestinal failure in a mouse model of congenital tufting enteropathy.

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Syndromic congenital tufting enteropathy (CTE) is a life-threatening recessive human genetic disorder, which is caused by mutations in SPINT2, encoding the protease inhibitor, HAI-2, and is characterized by severe intestinal dysfunction. We recently reported the generation of a

Inhibition of protease-activated receptor 1 ameliorates intestinal radiation mucositis in a preclinical rat model.

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OBJECTIVE To determine, using a specific small-molecule inhibitor of protease-activated receptor 1 (PAR1) signaling, whether the beneficial effect of thrombin inhibition on radiation enteropathy development is due to inhibition of blood clotting or to cellular (PAR1-mediated) thrombin

Activation of protease activated receptor 2 by exogenous agonist exacerbates early radiation injury in rat intestine.

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OBJECTIVE Protease-activated receptor-2 (PAR(2)) is highly expressed throughout the gut and regulates the inflammatory, mitogenic, fibroproliferative, and nociceptive responses to injury. PAR(2) is strikingly upregulated and exhibits increased activation in response to intestinal irradiation. We

Isolation and characterization of alpha 1-protease inhibitor from canine plasma.

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OBJECTIVE To improve a previously described purification process by producing a higher yield and purity of alpha 1-protease inhibitor (alpha 1-PI) from canine plasma. METHODS Plasma pool from 10 clinically normal male dogs. METHODS Canine alpha 1-PI was purified by use of ammonium sulfate

Food hypersensitivity reactions in Soft Coated Wheaten Terriers with protein-losing enteropathy or protein-losing nephropathy or both: gastroscopic food sensitivity testing, dietary provocation, and fecal immunoglobulin E.

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The purpose of this study was to evaluate Soft Coated Wheaten Terriers (SCWTs) affected with protein-losing enteropathy (PLE) or protein-losing nephropathy (PLN) or both for allergy to food. We performed gastroscopic food-sensitivity testing, a provocative dietary trial, and measurement of fecal

Preliminary characterisation of extracellular serine proteases of Dermatophilus congolensis isolates from cattle, sheep and horses.

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Dermatophilus congolensis is a filamentous branching actinomycete that causes dermatophilosis, an exudative dermatitis in ruminants. The pathogenesis of this disease is poorly understood and virulence factors of D. congolensis have not been characterised. Culture filtrate (CF) of 14 D. congolensis

Loss of HAI-2 in mice with decreased prostasin activity leads to an early-onset intestinal failure resembling congenital tufting enteropathy.

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Prostasin (CAP1/PRSS8) is a glycosylphosphatidylinositol (GPI)-anchored serine protease that is essential for epithelial development and overall survival in mice. Prostasin is regulated primarily by the transmembrane serine protease inhibitor, hepatocyte growth factor activator inhibitor (HAI)-2,

Effect of cyclosporin A treatment on the enteropathy of graft-versus-host reaction in the rat: a quantitative study of intestinal morphology, epithelial cell kinetics and mucosal immune activity.

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Mucosal graft-versus-host reaction (GvHR) of the small intestine exemplifies an immunologically mediated enteropathy that is associated with expansion of mucosal mast cells (MMC). Quantitative measures of intestinal morphology, epithelial cell kinetics and mucosal immune activity were used to assess

Serglycin proteoglycans limit enteropathy in Trichinella spiralis-infected mice.

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Serglycin proteoglycans are essential for maturation of secretory granules and for the correct granular storage of cationic proteases in hematopoietic cells, e.g. mast cells. However, little is known about the in vivo functions of serglycin proteoglycans during infection. Here we investigated the

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