Bone marrow triglyceride accumulation and hormonal changes during long-term alcohol intake in male and female rats.
Fjalë kyçe
Abstrakt
BACKGROUND
Chronic alcohol consumption may influence the metabolism of adipocytes, the most abundant stromal cell phenotype in bone marrow, and promote bone marrow triglyceride accretion.
METHODS
Male and female rats 35 days old were fed the Lieber-De Carli liquid diet containing 36% of the calories as alcohol and were compared with pair-fed rats given an isocaloric liquid diet in which maltose-dextrin substituted for the calories supplied by alcohol. Other control rats were fed chow ad libitum. The rats were maintained on these diets for 64 days, after which the femurs were recovered and examined.
RESULTS
End weights of male and female alcohol-fed rats were significantly lower than both control groups. Femur diaphyseal bone marrow triglyceride levels were significantly increased in alcohol-fed male and female rats compared with both control groups. Femur bone marrow cavity diameters were significantly increased and cortical thickness was significantly decreased by alcohol in both males and females. Serum insulin levels were significantly decreased by alcohol only in female rats compared with the ad libitum but not the pair-fed control group, and insulin-like growth factor-1 levels were significantly reduced in male and female rats given the alcohol diet compared with both controls. Male testosterone and female estradiol levels remained unchanged. Male estradiol levels were significantly increased by alcohol compared with both controls, and female progesterone levels were significantly reduced by alcohol compared with pair-fed rats. Whereas female leptin levels were unchanged by alcohol, male leptin levels were significantly increased by alcohol compared with pair-fed rats.
CONCLUSIONS
Hormonal and growth factor changes during chronic alcohol consumption accompany triglyceride accumulation in diaphyseal bone marrow and may parallel the effects of alcohol on mesenchymal stem cells and the balance between osteogenic and adipogenic lineages and their cellular progenies.
