Synergistic antibacterial efficacy of biogenic synthesized silver nanoparticles using Ajuga bractosa with standard antibiotics: a study against bacterial pathogens.

Multi-drug resistance in bacterial pathogens is a rising concern today. Green synthesis technology has been utilized to cure infectious diseases.The aim of the current research was to highlight the antibacterial, antioxidant, and phytochemical screening of green synthesized silver nanoparticles using Ajuga bracteosa.Extract of A. bracteosa was prepared by maceration technique. Silver nanoparticles were synthesized using A. bracteosa extract and were confirmed by UV-vis spectrophotometer, Scanning electron microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). The antibacterial, anti-biofilm, cell proliferation inhibition, TLC-Bioautography, TLC-Spot screening, antioxidant, and phytochemical screening were also investigated.UV-viz spectrum and Scanning electron microscopy indicated the synthesis of green nanoparticles at 400 nm with tube like structures. FTIR spectrum showed the functional groups have a role in capping and stability of AgNP. Agar well diffusion assay represents the maximum antibacterial effect of ABAgNPs against Escherichia coli, Klebsiella pneumoniae, Streptococcus pyogenes, Staphylococcus aureus, and Pseudomonas aeruginosa at 0.10 g/ml concentration compared to ABaqu. Two types of interactions among nanoparticles, aqueous extract, and antibiotics (Synergistic and additive) were recorded against tested pathogens. Crystal violet, MTT, TLC-bio-autography, and spot screening supported the findings of antibacterial assay. Highest antioxidant potential effect in ABaqu 14.62% (DPPH) and 13.64% (ABTS) while 4.85% (DPPH) and 4.86% (ABTS) was recorded in ABAgNPs. Presence of phytochemical constituents showed pharmacological importance.It has been concluded that green synthesis is an innovative technology in which natural products are used against infectious pathogens. Current research shows the significant antibacterial effect against infectious agents.