Tumoricidal function against human cervical-cancer cells in human peritoneal mesothelial cells - contribution of tumor-necrosis-factor-alpha.
Fjalë kyçe
Abstrakt
The present study demonstrates the important and novel finding that human mesothelial cells possess tumoricidal activity. This cytotoxicity in mesothelial cells was induced by recombinant human interferon (rhIFN)-gamma and was measured against the human cervical carcinoma cell line, ME-180, by Cr-51-release, visual assessment and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide uptake assays. Tumor necrosis factor (TNF)-alpha-sensitive ME-180 cells were shown to be lysed by 24-h-rhIFN-gamma-treated-mesothelial cell-supernatants alone, which was partially inhibitable by anti-TNF-alpha. Induction of TNF-alpha in mesothelial cells was confirmed by Northern blotting. Our data describe a novel rhIFN-gamma-activated cytotoxic function in mesothelial cells, and suggest the involvement of TNF-alpha as one of the lytic mechanisms. These results are of extreme importance in relation to the future understanding of general local carcinogenic processes, and henceforth therapies.