phosphoglucomutase/duhani

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Identification of the 64 kilodalton chloroplast stromal phosphoprotein as phosphoglucomutase.

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Identifikohuni Regjistrohu

Phosphorylation of the 64 kilodalton stromal phosphoprotein by incubation of pea (Pisum sativum) chloroplast extracts with [gamma-(32)P]ATP decreased in the presence of Glc-6-P and Glc-1,6-P(2), but was stimulated by glucose. Two-dimensional gel electrophoresis following incubation of intact

A survey for isoenzymes of glucosephosphate isomerase, phosphoglucomutase, glucose-6-phosphate dehydrogenase and 6-Phosphogluconate dehydrogenase in C3-, C 4-and crassulacean-acid-metabolism plants, and green algae.

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Identifikohuni Regjistrohu

Two isoenzymes each of glucosephosphate isomerase (EC 5.3.1.9), phosphoglucomutase (EC 2.7.5.1), glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (EC 1.1.1.43) were separated by (NH4)2SO4 gradient solubilization and DEAE-cellulose ion-exchange chromatography from

A Starchless Mutant of Nicotiana sylvestris Containing a Modified Plastid Phosphoglucomutase.

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Identifikohuni Regjistrohu

A mutant (NS 458) of Nicotiana sylvestris (Spegazzini and Comes) unable to synthesize leaf starch was isolated in the M(2) generation following ethyl methanesulfonate mutagenesis by testing with iodine. Segregation ratios in reciprocal F(2) progenies showed that the starchless phenotype resulted

Expression of Arabidopsis plastidial phosphoglucomutase in tobacco stimulates photosynthetic carbon flow into starch synthesis.

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Identifikohuni Regjistrohu

Phosphoglucomutase (PGM, EC 2.7.5.1) is one of the enzymes constituting the carbohydrate synthesis pathway in higher plants. It catalyzes the reversible conversion of glucose 6-phosphate (Glc6P) to glucose 1-phosphate (Glc1P). Previously, metabolic turnover analysis using (13)CO(2) in tobacco leaves

Chloroplast DNA assorts randomly in intraspecific somatic hybrids of Nicotiana debneyi.

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Identifikohuni Regjistrohu

Plants were regenerated following intraspecific fusion of leaf protoplasts from two naturally occurring genotypes of Nicotiana debneyi. The two genotypes differed in the EcoRl fragmentation pattern of chloroplast DNA and in the nuclear-coded phosphoglucomutase (Pgm) isozymes. There was no conscious

Influence of starch deficiency on photosynthetic and post-photosynthetic carbon isotope fractionations.

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Identifikohuni Regjistrohu

Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2. However, responses of 13C fractionations to diel variation in starch metabolism in the leaf are not fully understood. Here we

Reduced gravitropic sensitivity in roots of a starch-deficient mutant of Nicotiana sylvestris.

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Identifikohuni Regjistrohu

Gravitropism was studied in seedlings of Nicotiana sylvestris Speg. et Comes wild-type (WT) and mutant NS 458 which has a defective plastid phosphoglucomutase (EC 2.7.5.1.). Starch was greatly reduced in NS 458 compared to the WT, but small amounts of starch were detected in rootcap columella cells

Steady-State and Oscillating Photosynthesis by a Starchless Mutant of Nicotiana sylvestris.

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Identifikohuni Regjistrohu

The photosynthetic characteristics of wild type Nicotiana sylvestris (Speg. et Comes) were compared with those of a ;starch-less' mutant NS458 that contains a defective plastid phosphoglucomutase (EC 2.1.5.1) (KR Hanson, NA McHale [1988] Plant Physiol 88: 838-844). The steady-state rate of net CO(2)

Carbon Partitioning and Growth of a Starchless Mutant of Nicotiana sylvestris.

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Identifikohuni Regjistrohu

We have further characterized the photosynthetic carbohydrate metabolism and growth of a starchless mutant (NS 458) of Nicotiana sylvestris that is deficient in plastid phosphoglucomutase (Hanson KR, McHale NA [1988] Plant Physiol 88: 838-844). In general, the mutant had only slightly lower rates of

Evidence for Mitochondrial Regulation of Photosynthesis by a Starchless Mutant of Nicotiana sylvestris.

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Identifikohuni Regjistrohu

Mutant NS458 of Nicotiana sylvestris (Speg. et Comes) contains a defective plastid phosphoglucomutase and accumulates only trace amounts of starch. Determinations of carbon partitioning using tracer d-[3-(14)C]glyceric acid showed that the maximal CO(2) assimilation by mature leaves of the mutant at

A Nuclear Mutation in Nicotiana sylvestris Causing a Thiamine-Reversible Defect in Synthesis of Chloroplast Pigments.

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Identifikohuni Regjistrohu

We report the recovery of a nuclear recessive mutation in Nicotiana sylvestris (Spegazzini and Comes) producing a conditional disruption in the pathway for synthesis of chlorophyll a and b and carotenoids which is fully reversible by exogenous thiamine (0.3 micromolar). In the absence of

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