Optimized Extraction of Polysaccharides from Bergenia emeiensis Rhizome, Their Antioxidant Ability and Protection of Cells from Acrylamide-induced Cell Death

Bergeniaemeiensis is a traditional herb in Chinese folk medicine. Most related studies are focused on the bioactivity of bergenin, neglecting other bioactive compounds. In our previous work, polysaccharides were identified in B. emeiensis rhizome. To evaluate the extraction process and the antioxidant ability of these polysaccharides, a response surface method and antioxidant assays were applied. The results showed that the yield of polysaccharides was highly affected by extraction time, followed by temperature and solvent-to-sample ratio. Under the optimal conditions (43 °C, 30 min and 21 mL/g), the yield was 158.34 ± 0.98 mg/g. After removing other impurities, the purity of the polysaccharides from B. emeiensis (PBE) was 95.97 ± 0.92%. The infrared spectrum showed that PBE had a typical polysaccharide structure. Further investigations exhibited the PBE could scavenge well DPPH and ABTS free radicals and chelate Fe²⁺, showing an excellent antioxidant capacity. In addition, PBE also enhanced the cell viability of HEK 239T and Hep G2 cells under acrylamide-exposure conditions, exhibiting great protection against the damage induced by acrylamide. Therefore, PBE can be considered a potential natural antioxidant candidate for use in the pharmaceutical industry as a health product.

Keywords: Bergenia emeiensis; RSM; acrylamide-induced damage; optimization; polysaccharides.