Systematic approach of chromone skeleton for detecting Mg2+, ion: Applications for sustainable cytotoxicity and cell imaging possibilities.

The systematic studies of chromone appended novel chemosensors, favored to Mg²⁺ ion detection, these were analyzed and characterized by different spectroscopic techniques such as NMR, mass spectroscopy, FTIR and optical techniques. The binding demeanor of the ligands was executed with the library of metal ions and shown the good coordination with Mg²⁺ ion to the ligand's cavity. Both ligands demonstrated good binding behavior with Mg²⁺ ion. The ligands represented 1: 1 stoichiometry with Mg²⁺ ions through Job's plot. The low limit of detection of Mg²⁺ ion was determined as 2.56 × 10^-6 and 1.28 × 10^-6 for L_a and L_b respectively. No interference was occurred in Inference study by foreign metal ions that supported the specific detection of Mg²⁺ ion among the other metal ions. Further, the cytotoxicity assay test of these chromone appended ligands revealed that both ligands and their respective compound with Mg²⁺ ion shown negligible toxicity with HeLa cancer cell line. Further, due to the fluorescence properties of the ligands, with or without Mg²⁺ ion was successfully tested in bioimaging experiment of HeLa cancer cell lines and found that ligands with Mg²⁺ ions represented good imaging with HeLa cancer cell.