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Страна 1 од 62 резултати
Photoreactivation of tobacco mosaic virus and potato virus X ribonucleic acids inactivated by acetone-sensitized photoreaction.
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[Role of lipids in the function of microsomal electron transport chains of potato].
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Microsomal membranes from potato tubers were extracted by acetone solutions of increasing concentrations (5, 10, 15, 20, 30, 40, 50, 70 and 90 p. cent). Microsomal lipids were progressively extracted: acetone concentrations exceeding 30 p. cent extracted large amounts of membraneous phospholipids
The hatching factor of the potato-root eelworm.
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1. The hatching factor of the potato-root eelworm was concentrated from potato-root leachings by adsorption on charcoal. The crude material, extracted from charcoal with acetone, was purified by partition between ethyl acetate and m-potassium dihydrogen phosphate, and ethyl acetate and a solution of
High pressure liquid chromatography with ultraviolet absorbance or fluorescence detection of carbaryl in potato and corn.
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Carbaryl (1-naphthyl-N-methylcarbamate) was extracted from corn and potato with acetone. The acetone extract was partitioned into methylene chloride-hexane and concentrated for cleanup on a 5% water-deactivated Florisil column. The fraction containing the carbamate was subjected to high pressure
[Methodological hints on the gas chromatographic determination of kelevan and kepone in potatoes, potato foliage and soil].
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The gas chromatographic determination of Kelevan, an ethyllaevulinic acid derivative of Kepone, requires its conversion to Kepone. Thermal decomposition in the injection block of the gas chromatograph at 45 degrees C. has proved to be well suited for routine work. The sample is extracted with
Purification and properties of a lipid acyl-hydrolase from potato tubers.
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1. A pure lipid acyl-hydrolase was prepared from potato tubers by acetone precipitation, Sephadex G-100 and DEAE-Sephadex A-50 column chromatography, and by electrofocusing. 2. The purified enzyme was an acidic protein of pI 5.0 and molecular weight of about 70 000. Km values were 0.38 mM for
Isolation and properties of lysosomes from dark-grown potato shoots.
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A method is described for the isolation of lysosomal fractions from dark-grown potato shoots using a single stage separation on a Ficoll gradient. Peaks of acid hydrolase activity consisting of acid phosphatase, phosphodiesterase, ribonuclease, carboxylic esterase and β-glycerophosphatase were well
Acetone-butanol-ethanol production from substandard and surplus dates by Egyptian native Clostridium strains.
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One hundred and seven mesophilic isolates of Clostridium were isolated from agricultural soils cultivated with different plants in Assuit Governorate, Egypt. Eighty isolates (out of 107) showed the ability to produce ABE (Acetone, butanol and ethanol) on T6 medium ranging from 0.036 to 31.89 g/L.
Invertase inhibitors from sweet potato (Ipomoea batatas): purification and biochemical characterization.
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Two proteinaceous invertase inhibitors, designated ITI-L and ITI-R, were purified to electrophoretic homogeneity. ITI-L was purified from acetone powder of sweet potato leaves through sequential steps entailing buffer extraction, acid treatment, DEAE-Sephacel ion-exchange chromatography, and
Screening, separating, and completely recovering polyphenol oxidases and other biochemicals from sweet potato wastewater in starch production.
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Polyphenol oxidase (PPO) has multiple functions, and the lack of commercially available enzyme sources limits its widespread application in various industries. An accurate PPO assay was developed by HPLC determination of the substrate oxidation. Resources screening indicated that sweet potato
Mechanism of Chilling Injury in Sweet Potato: X. Change in Lipid-Protein Interaction in Mitochondria from Cold-stored Tissue.
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Seventy per cent of the phospholipid in mitochondria from sweet potato roots was removed by aqueous acetone treatment. The amount of phospholipid that could be rebound to these lipid-depleted mitochondria roughly corresponded to the amount of phospholipid in untreated mitochondria. The activities of
Bacillus subtilis BS 107 as an antagonist of potato blackleg and soft rot bacteria.
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Antimicrobial substances were produced by Bacillus subtilis BS 107 in a defined medium and isolated from culture filtrate by precipitation at pH 2.5. Active fractions were extracted in ethyl acetate, acetone, and 80% ethanol and purified by thin-layer chromatography (TLC) on silica gel plates
The fermentative production of acetone-butanol by Clostridium acetobutylicum.
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Fourteen different media were used in the fermentative production of acetone-butanol. The highest total yields were achieved in medium I. Potato starch and soluble starch were suitable as carbon sources. The best concentrations of potato starch and soluble starch were 500.0 and 10.0 g/l,
Leaf surface extracts ofSolanum berthaultii hawkes deter colorado potato beetle feeding.
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Leaf rinses ofS. berthaultii PI 473334 with methylene chloride were deterrent to feeding by the Colorado potato beetle when applied toS. tuberosum tuber and leaf disks. When the leaf rinse was separated into its nonvolatile and volatile fractions and applied to tuber disks, the nonvolatile fraction
Purification and characterization of a trypsin inhibitor from Solanum tuberosum.
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A trypsin inhibitor isolated from a potato acetone powder has been purified by affinity chromatography. This protein inhibits trypsin mole per mole. To a lesser extent it combines also with chymotrypsin and elastase. For trypsin, K1 = 8 X 10(-7) M. The inhibitor has a single polypeptide chain of 207
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