actinomycosis/carbohydrate

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Metabolic Profile of the Cellulolytic Industrial Actinomycete Thermobifida fusca.

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Actinomycetes have a long history of being the source of numerous valuable natural products and medicinals. To expedite product discovery and optimization of biochemical production, high-throughput technologies can now be used to screen the library of compounds present (or produced) at a given time

Binding of bivalent ions to actinomycete mannanase is accompanied by conformational change and is a key factor in its thermal stability.

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The study aimed to define the key factors involved in the modulation of actinomycete mannanases. We focused on the roles of carbohydrate-binding modules (CBMs) and bivalent ions. To investigate the effects of these factors, two actinomycete mannanase genes were cloned from Streptomyces thermoluteus

Analysis of coenzyme A activated compounds in actinomycetes.

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Acyl-CoAs are crucial compounds involved in essential metabolic pathways such as the Krebs cycle and lipid, carbohydrate, and amino acid metabolisms, and they are also key signal molecules involved in the transcriptional regulation of lipid biosynthesis in many organisms. In this study, we took

Antibacterial activity of Pseudonocardia sp. JB05, a rare salty soil actinomycete against Staphylococcus aureus.

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Staphylococcus aureus is a Gram-positive bacterium that causes many harmful and life-threatening diseases. Some strains of this bacterium are resistant to available antibiotics. This study was designed to evaluate the ability of indigenous actinomycetes to produce antibacterial compounds against S.

Lactose-reversible coaggregation between oral actinomycetes and Streptococcus sanguis.

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Freshly isolated strains of oral actinomycetes were obtained from human dental plaque and were tested for the ability to coaggregate with common laboratory stock strains of Streptococcus sanguis. Strains belonging to the genera Actinomyces, Arachnia, Bifidobacterium, and Bacterionema were isolated.

Macroamphiphilic components of thermophilic actinomycetes: identification of lipoteichoic acid in Thermobifida fusca.

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The cell envelopes of gram-positive bacteria contain structurally diverse membrane-anchored macroamphiphiles (lipoteichoic acids and lipoglycans) whose functions are poorly understood. Since regulation of membrane composition is an important feature of adaptation to life at higher temperatures, we

Prevalence of viridans streptococci exhibiting lactose-inhibitable coaggregation with oral actinomycetes.

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Fresh oral isolates from human dental plaque were selected on the basis of their spherical morphology. In a double-blind experiment, their species identity and ability to coaggregate with human oral Actinomyces viscosus and Actinomyces naeslundii were determined. Of the 110 isolates characterized,

Computed tomography for the preoperative diagnosis of pelvic actinomycosis.

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OBJECTIVE The aim of this study was to review the clinical manifestations of pelvic actinomycosis in order to aid clinicians in its diagnosis and treatment. METHODS We retrospectively reviewed the histopathology and clinical records of all cases confirmed by surgery as pelvic actinomycosis from 1996

[Purification and properties of beta-D-mannanase from Nocardioform actinomycetes].

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After cultivating in liquid medium for 3 days, an extracellular endo-1,4-beta-D-mannanase (EC-3.2.1.78) from a Nocardioform actinomycetes strain NA3-540 was purified 137-fold to electrophoretic homogeneity by ammonium sulfate precipitation, 95% alcohol precipitation, CM-sephadex A-50, hydroxyaptite

Genome sequence and analysis of the soil cellulolytic actinomycete Thermobifida fusca YX.

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Thermobifida fusca is a moderately thermophilic soil bacterium that belongs to Actinobacteria. It is a major degrader of plant cell walls and has been used as a model organism for the study of secreted, thermostable cellulases. The complete genome sequence showed that T. fusca has a single circular

Terrabacter terrae sp. nov., a novel actinomycete isolated from soil in Spain.

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A Gram-positive, aerobic, long-rod-shaped, non-spore-forming bacterium (strain PPLB(T)) was isolated from soil mixed with Iberian pig hair. This actinomycete showed keratinase activity in vitro when chicken feathers were added to the culture medium. Strain PPLB(T) was oxidase-negative and

Nitrogen regulator GlnR controls uptake and utilization of non-phosphotransferase-system carbon sources in actinomycetes.

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The regulatory mechanisms underlying the uptake and utilization of multiple types of carbohydrates in actinomycetes remain poorly understood. In this study, we show that GlnR (central regulator of nitrogen metabolism) serves as a universal regulator of nitrogen metabolism and plays an important,

Transcriptome analysis of Valsa mali reveals its response mechanism to the biocontrol actinomycete Saccharothrix yanglingensis Hhs.015.

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BACKGROUND Apple canker is a devastating branch disease caused by Valsa mali (Vm). The endophytic actinomycete Saccharothrix yanglingensis Hhs.015 (Sy Hhs.015) can effectively inhibit the growth of Vm. To reveal the mechanism, by which Vm respond to Sy Hhs.015, the transcriptome of Vm was analyzed

Isolation and in vitro selection of actinomycetes strains as potential probiotics for aquaculture.

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OBJECTIVE This study was designed to describe a series of in vitro tests that may aid the discovery of probiotic strains from actinomycetes. METHODS Actinomycetes were isolated from marine sediments using four different isolation media, followed by antimicrobial activity and toxicity assessment by

Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography.

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A system has been developed for the identification of aerobic actinomycetes in the clinical laboratory based on analysis of whole cells for diaminopimelic acid and carbohydrates and on the ability of the organism to decompose casein, tyrosine, and xanthine media. The whole-cell analyses were

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