daucus sativus/phosphatase

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Ultrastructural localization of acid phosphatase in cultured cells ofDaucus carota.

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Acid phosphatase localization has been studied, using the lead salt method, in suspension-cultured cells of the wild carrot. Enzyme activity in most of the cells was restricted to the walls and vacuoles. However, in some senescent cells activity was also seen in the nucleus, at one face of the

Regulation of ascorbic acid biosynthesis and recycling during root development in carrot (Daucus carota L.).

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Ascorbic acid (AsA), also known as vitamin C, is an essential nutrient in fruits and vegetables. The fleshy root of carrot (Daucus carota L.) is a good source of AsA for humans. However, the metabolic pathways and molecular mechanisms involved in the control of AsA content during root development in

[Characterization of root initiation and root senescence in callus- and organ-cultures of Daucus carota through determination of the specific activity of the glutamate-dehydrogenase (NAD)].

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In callus tissues of Daucus carota rhizogenesis was influenced by addition to the cultures of solutions with different kinetin concentrations (0-0.1-0.5-2 mg/l) and a constant auxin content (1 mg/l). In these cultures the specific activity of glutamate-dehydrogenase (E.C. 1.4.1.2.),

Changes in phosphatidylinositol metabolism in response to hyperosmotic stress in Daucus carota L. cells grown in suspension culture.

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Carrot (Daucus carota L.) cells plasmolyzed within 30 s after adding sorbitol to increase the osmotic strength of the medium from 0.2 to 0.4 or 0.6 osmolal. However, there was no significant change in the polyphosphorylated inositol phospholipids or inositol phosphates or in inositol phospholipid

Hepatoprotective activity of carrot (Daucus carota L.) against carbon tetrachloride intoxication in mouse liver.

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The effect of carrot extract on carbon tetrachloride (CCl4)-induced acute liver damage was evaluated. The increased serum enzyme levels (viz., glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, lactate dehydrogenase, alkaline phosphatase, sorbitol and glutamate dehydrogenase) by

Glycerophosphocholine metabolism in higher plant cells. Evidence of a new glyceryl-phosphodiester phosphodiesterase.

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Glycerophosphocholine (GroPCho) is a diester that accumulates in different physiological processes leading to phospholipid remodeling. However, very little is known about its metabolism in higher plant cells. (31)P-Nuclear magnetic resonance spectroscopy and biochemical analyses performed on carrot

Overexpressing AtPAP15 enhances phosphorus efficiency in soybean.

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Low phosphorus (P) availability is a major constraint to crop growth and production, including soybean (Glycine max), on a global scale. However, 50% to 80% of the total P in agricultural soils exists as organic phosphate, which is unavailable to plants unless hydrolyzed to release inorganic

The genes ABI1 and ABI2 are involved in abscisic acid- and drought-inducible expression of the Daucus carota L. Dc3 promoter in guard cells of transgenic Arabidopsis thaliana (L.) Heynh.

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The ABA INSENSITIVE1 (ABI1) and ABI2 genes encode homologous type-2C protein phosphatases with redundant yet distinct functions in abscisic acid (ABA) responses. Results from Northern blot analysis showed that ABA- and mannitol-inducible expression of the COR47 and COR78/LTI78/RD29A (COR78) genes

In vivo antioxidant and hepatoprotective activity of methanolic extracts of Daucus carota seeds in experimental animals.

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OBJECTIVE To assess the In vivo antioxidFant and hepatoprotective activity of methanolic extract of Daucus carota (D. carota) seeds in experimental animals. METHODS Methanolic extracts of D. carota seeds is used for hepatoprotection assessment. Oxidative stress were induced in rats by thioacetamide

Biochemical differences between carrot inbreds differing in plant regeneration potential.

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Cultures derived from domestic carrot (Daucus carota L.) inbreds were found to vary with respect to regeneration potential as measured by the production of somatic embryos in suspension cultures. A number of biochemical parameters previously reported to distinguish embryogenic from non-embryogenic

Purification of a trypsin inhibitor secreted by embryogenic carrot cells.

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A protease inhibitor with a molecular weight of about 12,800 was purified to electrophoretic homogeneity from Daucus carota cells. The protease inhibitor was heat stable and inhibited trypsin but had no activity toward chymotrypsin or subtilisin. Nonembryogenic as well as embryogenic strains

Isolation and identification of substances inducing formation of tracheary elements in cultured carrot-root slices.

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The roots of a carrot (Daucus carota L.) cultivar, Kuroda-gosun, contain active substances causing formation of tracheary elements in cultured root phloem slices. In the roots of another carrot cultivar, Hokkaidô-gosun, such substances could not be detected but when root-phloem slices of this

The influence of external nitrogen on carbon allocation to Glomus intraradices in monoxenic arbuscular mycorrhiza.

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The influence of external nitrogen (N) on carbon (C) allocation and processes related to phosphorus (P) metabolism were studied in monoxenic arbuscular mycorrhiza (AM) cultures of Daucus carota. Fungal hyphae of Glomus intraradices proliferated from colonized roots growing on solid medium into

Phosphorus effects on metabolic processes in monoxenic arbuscular mycorrhiza cultures.

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The influence of external phosphorus (P) on carbon (C) allocation and metabolism as well as processes related to P metabolism was studied in monoxenic arbuscular mycorrhiza cultures of carrot (Daucus carota). Fungal hyphae of Glomus intraradices proliferated from the solid minimal medium containing

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