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The effect of diarrhea on the oxidation of 14C-phenylalanine in piglets receiving diets varying in protein and proline concentration.

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The oxidation rate of L-[1-14C]phenylalanine in piglets suffering from diarrhea and receiving diets varying in concentration of protein or proline was compared to the oxidation rates in healthy animals. Phenylalanine oxidation was used to indicate the partition of absorbed amino acids between

Involvement of a bovine viral diarrhea virus NS5B locus in virion assembly.

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A novel mutant of bovine viral diarrhea virus (BVDV) was found with a virion assembly phenotype attributable to an insertion into the NS5B polymerase locus. This mutant, termed 5B-741, was engineered by reverse genetics to express NS5B with a C-terminal peptide tag of 22 amino acids. Electroporation

GC-MS and metabolomics analysis of amino acids, glucose and urinary metabolic pathways and characteristics in children with spleen-deficiency diarrhea

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Diarrhea is a disease, and patients must have bowel movements at least three times per day. This condition may last for several days and may cause dehydration due to fluid loss. Spleen-deficiency makes a person more vulnerable to some infectious diseases. Persistent diarrhea due to spleen-deficiency

Generation and characterization of an Npro-disrupted marker bovine viral diarrhea virus derived from a BAC cDNA.

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In vitro studies showed that N(pro) protein of bovine viral diarrhea virus (BVDV) interferes with cellular antiviral defense. To understand the role of N(pro) protein in successful viral invasion of the host and establishment of the lifetime persistence, an infectious N(pro)-disrupted virus with a

Structure of the extended diarrhea-inducing domain of rotavirus enterotoxigenic protein NSP4.

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Rotavirus nonstructural protein 4 (NSP4) is a multidomainal and multifunctional protein and is recognized as the first virus-encoded enterotoxin. Extensive efforts to crystallize the complete cytoplasmic tail (CT), which exhibits all the known biological functions, have been unsuccessful, and to

Clostridioides difficile uses amino acids associated with gut microbial dysbiosis in a subset of patients with diarrhea.

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The gut microbiota plays a critical role in pathogen defense. Studies using antibiotic-treated mice reveal mechanisms that increase susceptibility to Clostridioides difficile infection (CDI), but risk factors associated with CDI in humans extend beyond antibiotic use. Here, we studied the dysbiotic

Metabolomics analysis of herb-partitioned moxibustion treatment on rats with diarrhea-predominant irritable bowel syndrome.

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Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder, which is commonly treated with antidiarrhoeal, antispasmodics, serotonergic agents or laxative agents. These treatments provide relief for IBS symptoms but may also lead to undesired side effects.

Structural Basis of Proline-Proline Peptide Bond Specificity of the Metalloprotease Zmp1 Implicated in Motility of Clostridium difficile.

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Clostridium difficile is a pathogenic bacterium causing gastrointestinal diseases from mild diarrhea to toxic megacolon. In common with other pathogenic bacteria, C. difficile secretes proteins involved in adhesion, colonization, and dissemination. The recently identified Zmp1 is an extracellular

Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked Syndrome Associated With a Novel Mutation of FOXP3 Gene.

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The immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome is a rare, x-linked, recessive disorder characterized by dysfunction of the T regulatory (Treg) lymphocytes leading to autoimmune diseases. Herein we report a male patient with IPEX syndrome who presented with severe

Cryo-EM structure of the CFA/I pilus rod.

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Enterotoxigenic Escherichia coli (ETEC) are common agents of diarrhea for travelers and a major cause of mortality in children in developing countries. To attach to intestinal cells ETEC express colonization factors, among them CFA/I, which are the most prevalent factors and are the

Rearrangement of the VP6 gene of a group A rotavirus in combination with a point mutation affecting trimer stability.

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A group A rotavirus isolated from a lamb with diarrhea in Qinhai province, China, was serially passaged in fetal calf kidney cells. In passage 96, rearrangements of RNA segments 5 and 6 of the viral genome were found. Here we report the nucleotide and predicted amino acid sequences of normal and

Utilization of carbon sources by clinical isolates of Aeromonas.

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Bacteria in the genus Aeromonas are primarily aquatic organisms; however, some species can cause diseases in humans, ranging from wound infections to septicemia, of which diarrhea is the most common condition. The ability to use a variety of carbon substrates is advantageous for pathogenic bacteria.

Secretory and GM1 receptor binding role of N-terminal region of LTB in Vibrio cholerae.

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Heat labile enterotoxin from enterotoxigenic Escherichia coli is similar to cholera toxin (CT) and is a leading cause of diarrhea in developing countries. It consists of an enzymatically active A subunit (LTA) and a carrier pentameric B subunit (LTB). In the current study, we evaluated the

Molecular and phylogenetic characterization of bovine coronavirus virus isolated from dairy cattle in Central Region, Thailand.

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Bovine coronavirus (BCoV) is involved mainly in enteric infections in cattle. This study reports the first molecular detection of BCoV in a diarrhea outbreak in dairy cows in the Central Region, Thailand. BCoV was molecularly detected from bloody diarrheic cattle feces by using nested PCR. Agarose

Mutations selected in rotavirus enterotoxin NSP4 depend on the context of its expression.

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The rotavirus NSP4 protein is cytotoxic when transiently expressed in cells and is capable of inducing secretory diarrhea in neonatal mice. NSP4 consists of 175 amino acids, and sequences important for its toxic effects have been mapped to the carboxy-terminal half of the protein. In this report, we

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