Screening of nutrient parameters for mevastatin production by Penicillium citrinum MTCC 1256 under submerged fermentation using the Plackett-Burman design.

OBJECTIVE

Mevastain, an HMG-CoA reductase inhibitor produced by Penicillium citrinum. The Placket-Burman experimental design was used to identify the important nutrients influencing the production of mevastatin by Penicillium citrinum MTCC 1256 under submerged fermentation.

METHODS

Nine nutritional parameters, such as, glucose, glycerol, arrowroot, oats, urea, peptone, yeast extract, MgSO(4).7H(2)O, and CaCl(2).2H(2)O were screened by Placket-Burman experimental design in 12 experimental run.

RESULTS

Nine nutritional parameters, glucose, glycerol, arrowroot, oats, urea, peptone, yeast extract, MgSO(4).7H(2)O, and CaCl(2).2H(2)O contributed with 0.8114%, 24.0390%, 2.2786%, 0.1491%, 0.5608%, 47.5029%, 6.8092 %, 4.0980%, 10.5823% respectively towards mevastatin production by Penicillium citrinum under submerged fermentation.

CONCLUSIONS

Among the nine nutrient components glycerol, peptone, yeast extract, MgSO(4).7H(2)0, and CaCl(2).2H(2)O contributed to a large extent, urea had little impact, while glucose, oats, and arrowroot contributed moderately on production of mevastatin.