[Therapeutic Material Basis of Chemical Subdivisions of Anemarrhenae Rhizoma on Anti-inflammatory and Immunomudulatory Effects].

OBJECTIVE

To study the relationship between chemical subdivision and immune suppressive activity in order to find out the anti-inflammatory and immunomudulatory pharmacological activity material basic of Anemarrhenae Rhizoma.

METHODS

C57 mice was induced by 2,4-Dinitrofluorobenzene to build immune inflammation which was also called contact allergic dermatitis. The influence of Anemarrhenae Rhizoma decoction and chemical subdivisions on immune organ, ear edema and the Th1/Th2 immune balance was measured by analytical balance and Elisa method. The inflammatory factor TNF-α and NO level excreted by macrophage RAW264. 7 induced by LPS were also investigated.

RESULTS

AA decoction, timosaponin and polysaccharides significantly reduced the immune organ index and ear edema degree (P < 0.05), protein expression of IFN-γ was inhibited by AA timosaponin fraction and polysaccharides fraction. In vitro experiments showed that the proliferation of spleen cells was inhibited by timosaponin and polysaccharides after induced by ConA (P < 0.05). The release of NO and TNF-α induced by LPS significantly decreased by Anemarrhenae Rhizoma decoction and timosaponin (P < 0.05).

CONCLUSIONS

The significant anti-inflammatory and immunomudu latory effects of AA are related to timosaponin and polysaccharides.