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12 resultat
Effect of Na+ and K+ channel blockade on baseline and anoxia-induced catecholamine release from rat carotid body.
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Ionic membrane currents are hypothesized to play a major role in determining secretion from carotid body glomus cells, and increased secretion likely mediates the increase in nerve activity in response to hypoxia. The hypothesis that Na+ and K+ channels play an important role in determining
Adenosine A1 antagonism increases specific synaptic forms of glutamate release during anoxia, revealing a unique source of excitation.
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The role of the adenosine A1 receptor in the modulation of anoxia-induced synaptic glutamate release was examined in CA1 pyramidal neurons by whole-cell voltage-clamp recording in the rat hippocampal slice preparation. Anoxia leads to an increased action potential-independent synaptic glutamate
Effects of anoxia on rat midbrain dopamine neurons.
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1. Dopamine-containing neurons of the rat midbrain were recorded intracellularly in vitro. Anoxia (2-5 min) caused reversible membrane hyperpolarization (4-25 mV), which blocked spontaneous firing of action potentials. Under voltage clamp, anoxia produced an outward current (100-1,000 pA) associated
Effects of myocardial hypoxia and ischemia on myocardial scintigraphy.
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The effect of regional myocardial ischemia and hypoxia on myocardial scintigraphy was studied in patients and dogs after intravenous administration of cesium-129. Seven men with angiographically proved ischemic heart disease underwent exercise testing and 129Cs was given immediately when ischemia
Demonstration of a pump-mediated efflux in the epithelial potassium active transport system of insect midgut.
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The larval midgut epithelium of lepidopteran insects (e.g., Hyalophora cecropia and Manduca sexta) actively transports potassium from hemolymph to lumen when mounted in a chamber. The potassium active transport is rheogenic and does not require the presence of other alkali ions. The transepithelial
Responses of rat mesencephalic dopaminergic neurons to a prolonged period of oxygen deprivation.
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We employed intracellular electrophysiological techniques to examine the effects of a prolonged anoxia (more than 7 min superfusion with artificial cerebrospinal fluid saturated with 95% N2-5% O2) on dopaminergic neurons of the rat ventral mesencephalon maintained in vitro. A prolonged anoxia caused
Gamma irradiation induces acetylcholine-evoked, endothelium-independent relaxation and activates K-channels of isolated pulmonary artery of rats.
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OBJECTIVE
To test the effects of irradiation (R*) on the pulmonary artery (PA).
METHODS
Isolated PA rings were submitted to gamma irradiation (cesium, 8 Gy/min(-1)) at doses of 20 Gy-140 Gy. Rings were placed in an organ chamber, contracted with serotonin (10(-4) M 5-hydroxytryptamine [5-HT]), then
Functional hepatocyte cation compartmentation demonstrated with 133Cs NMR.
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This study utilized the large intrinsic chemical shift range of (133)Cs, a potassium congener, in an NMR study of intracellular cation distribution. It demonstrates two distinct intracellular environments in isolated perfused hepatocytes from cesium-fed rats, evident as compartments with different
Role of adenosine on ventricular overdrive suppression in isolated guinea pig hearts and Purkinje fibers.
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The present study was undertaken to demonstrate and characterize potentiation of ventricular overdrive suppression by adenosine. To substantiate that adenosine has an enhanced effect on overdrive suppression, it would be necessary to demonstrate that adenosine increases pause duration independent of
A Chinese Medicine Formula "Xian-Jia-Tang" for Treating Bladder Outlet Obstruction by Improving Urodynamics and Inhibiting Oxidative Stress through Potassium Channels.
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The aim of this study is to investigate efficacy of a traditional Chinese medicine formula (named Xian-Jia-Tang, XJT) on bladder outlet obstruction (BOO) in rats and explore its mechanisms. Total 80 BOO model rats were established and randomly divided into 4 groups: physiological saline, XJT, Cesium
Effect of metabolic inhibition on the excitability of isolated hippocampal CA1 neurons: developmental aspects.
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1. The effects of brief exposures to hypoxia on the membrane currents of isolated hippocampal CA1 neurons were studied with the use of the whole-cell variation of the patch-clamp technique. Neurons were acutely dissociated from immature (day 2-7) and mature (day 21-43) rats. 2. In the current-clamp
Transplanted senescent renal scattered tubular-like cells induce injury in the mouse kidney.
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Cellular senescence, a permanent arrest of cell proliferation, is characterized by a senescence-associated secretory phenotype (SASP), which reinforces senescence and exerts noxious effects on adjacent cells. Recent studies suggest that transplanting small numbers of senescent cells suffices to
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