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Evolutionary shifts to self-fertilisation restricted to geographic range margins in North American Arabidopsis lyrata.

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Cross-fertilisation predominates in eukaryotes, but shifts to self-fertilisation are common and ecologically and evolutionarily important. Reproductive assurance under outcross gamete limitation is one eco-evolutionary process held responsible for the shift to selfing. Although small effective

Identification of new members of Fertilisation Independent Seed Polycomb Group pathway involved in the control of seed development in Arabidopsis thaliana.

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In higher plants, double fertilisation initiates seed development. One sperm cell fuses with the egg cell and gives rise to the embryo, the second sperm cell fuses with the central cell and gives rise to the endosperm. The endosperm develops as a syncytium with the gradual organisation of domains

[The relationship between the conception of the role of histones in the mutagenic process and the combined effect of trichloracetic acid and dimethylsulfate on Arabidopsis].

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Separate and combined effect of TChAA and DMS was studied as applied to Arabidopsis of Estland race. When the Arabidopsis seeds were treated with TChAA in M1 separately, stimulation was observed as to certain features. A combined treatment with TChAA + DMS reduces significantly the yield of

Influence of mutation rate on estimators of genetic differentiation--lessons from Arabidopsis thaliana.

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BACKGROUND The analysis of molecular variation within and between populations is crucial to establish strategies for conservation as well as to detect the footprint of spatially heterogeneous selection. The traditional estimator of genetic differentiation (F(ST)) has been shown to be misleading if

New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen.

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Pollen tube growth is essential for plant reproduction. Their rapid extension using polarized tip growth provides an exciting system for studying this specialized type of growth. Self-Incompatibility (SI) is a genetically controlled mechanism to prevent self-fertilisation. Mechanistically, one of

Rootcap structure in wild type and in a starchless mutant of Arabidopsis.

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Rootcaps of the wild type (WT) and of a starchless, gravitropic mutant (TC7) of Arabidopsis thaliana L. were examined by electron microscopy to identify cellular polarities with respect to gravity. In columella cells, nuclei are located proximally, and the nuclear envelope is continuous with

An uncoupling screen for autonomous embryo mutants in Arabidopsis thaliana.

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Simple de novo screens in Arabidopsis thaliana have previously identified mutants that affect endosperm development but viable-embryo mutants have not been identified. Our strategy to identify autonomous embryo development was to uncouple embryo and endosperm fertilisation. This involved a

Arabidopsis thaliana, a plant Drosophila.

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Arabidopsis thaliana is a small cruciferous weed which grows naturally, mainly in Europe. Because of its qualities of small size, rapid growth, low chromosome number and self-fertilisation, I adapted it to aseptic growth in purified agar in sterile test-tubes. I found that it secreted various

MicroRNA and tasiRNA diversity in mature pollen of Arabidopsis thaliana.

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BACKGROUND New generation sequencing technology has allowed investigation of the small RNA populations of flowering plants at great depth. However, little is known about small RNAs in their reproductive cells, especially in post-meiotic cells of the gametophyte generation. Pollen - the male

How and when did Arabidopsis thaliana become highly self-fertilising.

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Changes in breeding system are a regular evolutionary change in plants, as self-fertilisation is often advantageous, particularly for weedy and colonising species. The adoption of Arabidopsis thaliana as a plant model species has led to interest in how self-incompatibility was lost so that this

Nuclear Chaperone ASF1 is Required for Gametogenesis in Arabidopsis thaliana.

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Sexual reproduction in flowering plants is distinct from that in animals since gametogenesis requires production of haploid spores, which divide and differentiate into specialised gametophyte structures. Anti-Silencing Function 1 (ASF1) is a histone H3/H4 chaperone involved in chromatin remodeling

NBS1 plays a synergistic role with telomerase in the maintenance of telomeres in Arabidopsis thaliana.

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BACKGROUND Telomeres, as elaborate nucleo-protein complexes, ensure chromosomal stability. When impaired, the ends of linear chromosomes can be recognised by cellular repair mechanisms as double-strand DNA breaks and can be healed by non-homologous-end-joining activities to produce dicentric

Morphology of rsw1, a cellulose-deficient mutant of Arabidopsis thaliana.

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The rsw1 mutant of Arabidopsis thaliana is mutated in a gene encoding a cellulose synthase catalytic subunit. Mutant seedlings produce almost as much cellulose as the wild type at 21 degrees C but only about half as much as the wild type at 31 degrees C. We used this conditional phenotype to

Sibling competition does not magnify inbreeding depression in North American Arabidopsis lyrata.

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About half of all angiosperms have some form of molecular self-incompatibility to promote outcrossing. If self-incompatibility breaks down, inbreeding depression (δ) is the main barrier to the evolution of self-fertilisation (selfing). If inbreeding depression is lower than 50% (δ < 0.5), the

Nitrogen as a key regulator of flowering in Fagus crenata: understanding the physiological mechanism of masting by gene expression analysis.

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The role of resource availability in determining the incidence of masting has been widely studied, but how floral transition and initiation are regulated by the resource level is unclear. We tested the hypothesis that floral transition is stimulated by high resource availabiltiy in Fagus crenata

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