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5 resultat
Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum.
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The FLP/FRT site-specific recombination system of Saccharomyces cerevisiae was expressed in stably transformed tobacco plants. The FLP protein efficiently catalyzes recombination between two directly repeated FLP recombination target (FRT) sites, deleting the sequence between them. In the constructs
Herbicides and the microtubular apparatus of Nicotiana tabacum pollen tube: immunofluorescence and immunogold labelling studies.
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Herbicides are chemical compounds widely used in agriculture. As their intensive application is becoming a cause of environmental pollution, detailed and more sophisticated investigations are needed to understand better their consequences at the biological level. After herbicides are dispersed in
Efficient male germ line transformation for transgenic tobacco production without selection.
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Microspores at late uninucleate/early binucleate stages were isolated from flower buds of tobacco (Nicotiana tabacum L.) and in vitro culture methods optimised for their maturation to fully functional viable pollen which, after application to the stigma of emasculated plants in situ, led to the
Characterisation of detergent-insoluble membranes in pollen tubes of Nicotiana tabacum (L.).
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Pollen tubes are the vehicle for sperm cell delivery to the embryo sac during fertilisation of Angiosperms. They provide an intriguing model for unravelling mechanisms of growing to extremes. The asymmetric distribution of lipids and proteins in the pollen tube plasma membrane modulates ion fluxes
Extracellular ionic fluxes suggest the basis for cellular life at the 1/f ridge of extended criticality.
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The criticality hypothesis states that a system may be poised in a critical state at the boundary between different types of dynamics. Previous studies have suggested that criticality has been evolutionarily selected, and examples have been found in cortical cell cultures and in the human nervous
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