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dental plaque/albumin

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Synergistic degradation of bovine serum albumin by mutans streptococci and other dental plaque bacteria.

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Mutans streptococci (Streptococcus mutans and Streptococcus sobrinus) exhibited low levels of proteolytic activity against the model protein substrate, FITC-labelled bovine serum albumin, when incubated alone. Inclusion of other members of the dental plaque microflora in the assay usually resulted

Effects of chlorhexidine on proteolytic and glycosidic enzyme activities of dental plaque bacteria.

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Chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by Treponema denticola, Porphyromonas gingivalis, Bacteroides intermedius, Actinobacillus actinomycemcomitans, Capnocytophaga sputigena, Capnocytophaga gingivalis, Capnocytophaga

Some factors affecting the diffusion of [14C]-lactate in human dental plaque.

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The apparent diffusion rate, D, of lactate was significantly retarded in dental plaque fluid and a simulated plaque fluid consisting of a chemically-defined solution of salts, amino acids and albumin in phosphate buffer at pH 6.5. Metabolic utilization of lactate in live plaque residue reduced D for

The adsorptive properties of hydroxyapatite to albumin, dextran and lipids.

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Hydroxyapatite is widely utilized as a component in dental hygiene agents. The ability to adsorb and remove dental plaque adhering to tooth surfaces is recognized as an important property of hydroxyapatite particles. In this study, the adsorptive ability of hydroxyapatite was evaluated based on its

alpha-Amylase and salivary albumin adsorption onto titanium, enamel and dentin: an in vivo study.

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In vivo adsorption of salivary albumin and alpha-amylase onto titanium, enamel and dentin was analysed following their exposure to the oral cavity for 2h. Oral appliances in six adults served as a platform for carrying 4-mm discs of the three materials. Adherent proteins were eluted from the discs

Proteolysis and utilization of albumin by enrichment cultures of subgingival microbiota.

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Subgingival dental plaque consists mainly of microorganisms that derive their energy from amino acid fermentation. Their nutrient requirements are met by the subgingival proteolytic system, which includes proteases from microorganism and inflammatory cells, and substrate proteins from sulcus

Flow and albumin content of early (pre-inflammatory) gingival crevicular fluid from human subjects.

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Gingival fluid was collected with glass capillaries tubing from the upper premolar area in a group of 7 volunteers, after allowing dental plaque to accumulate for 12-36 h, and in a group of patients with gingivitis. Whereas no fluid could be collected in the absence of plaque, increasing amounts
Albumin and alpha 2-macroglobulin were analysed in gingival fluid collected by filter paper strips from 6 volunteers before, during and after 10 days of dental plaque accumulation. The two proteins were also determined in samples of venous blood at the beginning of abstention from tooth brushing and

[Physicochemical properties of dental plaque proteins--electrophoretic mobility and molecular weight].

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In this work electrophoretic mobility as well as molecular weight of dental plaque proteins were examined. Three types of electrophoresis and three procedures for molecular weight estimation were applied. The results showed that the electrophoretic mobility of some protein fractions present in

The influence of albumin on corrosion resistance of titanium in fluoride solution.

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Proteins can interact with corrosion reactions in several ways. In this study, we investigated the effect of albumin on the corrosion resistance of titanium in the presence of fluoride. The effects of the NaF concentration, albumin concentration, and pH on the corrosion characteristics of

Specific and nonspecific immune factors in dental plaque fluid and saliva from young and old populations.

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Separate samples of supragingival dental plaque overtly free of blood were centrifuged to obtain the free fluid phase (plaque fluid). Bound protein was eluted from the plaque bacteria and matrix by washing the plaque with a low-pH buffer. The plaque fluid, low pH eluate, and whole saliva were

Studies on human dental plaque. 2. Immunochemical characteristics.

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From collected supragingival plaque, extracts were prepared for immunochemical analyses. Extracted sediments were examined by fluorescein-labeled antibodies for the presence of immunoglobulins. Precipitation with monospecific and polyvalent antisera revealed IgA, IgG, secretory component, C3,

Characterization of a trypsin-like protease from the bacterium Bacteroides gingivalis isolated from human dental plaque.

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A trypsin-like, membrane-bound protease from Bacteroides gingivalis was solubilized by Triton X-100 and partially purified by a combination of DEAE-Sepharose and aminophenylmercuric Sepharose chromatography, by taking advantage of the thiol group on the enzyme. The purified enzyme hydrolysed the

Adhesion of Actinomyces viscosus to Porphyromonas (Bacteroides) gingivalis-coated hexadecane droplets.

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Interbacterial adhesion (coadhesion) is considered a major determinant of dental plaque ecology. In this report, we studied several aspects of the adhesion of Porphyromonas (Bacteroides) gingivalis to hexadecane in order to use the liquid hydrocarbon as a convenient substratum for coadhesion assays.
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