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4 resultat
ATP-ADP compartmentation in storage pool deficient platelets: correlation between granule-bound ADP and the bleeding time.
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Platelets contain two major compartments of ATP and ADP, the cytosol and the dense granules. We separated the two compartments by controlled digitonin-induced cell lysis and measured both fractions directly in the platelets of 16 patients with storage pool deficiency. The total contents of ATP and
Glycoproteins V and Ib-IX form a noncovalent complex in the platelet membrane.
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Platelet glycoprotein (GP) V is a Mr 82,000 plasma membrane protein of unknown function that is cleaved by the potent platelet agonist, thrombin, to yield a Mr 69,500 fragment (GPVf1). Platelet GPIb, a disulfide-linked alpha beta heterodimer (Mr 160,000) that forms a noncovalent complex with GPIX
Comparison of the active calcium extrusion, calcium buffering capacity and ATPase activity in rabbit reticulocytes and mature red cells.
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The purpose of the present work was to study the changes in the pattern of calcium homeostasis following the loss of intracellular organella during red cell maturation. Reticulocytes and mature red cells were prepared from anaemic rabbits blood after daily bleeding. Experimental protocols were
Determination of residual erythrocytes in rat tissue homogenates using commercially available anti-red blood cell sera.
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A method to determine the residual erythrocyte content in rat tissue homogenates by means of radial immunodiffusion (Mancini et al., 1965) is described. The method makes use of commercially available antisera against rat erythrocytes. Tissues were homogenized after addition of digitonin and applied
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