This is a 3-week controlled isocaloric diet containing approximately 125 mg taurine, 40% of calories from fat, 15% of calories from saturated fat, 25% of calories from protein (4:1 animal to plant grams of protein), and 11.5 grams fiber/1000 calories.

This is a 3-week controlled isocaloric diet containing approximately 7 mg taurine, 36% of calories from fat, 8% of calories from saturated fat, 13% of calories from protein (3:1 plant to animal grams of protein), and 13.5 grams fiber/1000 calories.

Colorectal Cancer

This is a 3-week (21 day) isocaloric Western-type diet with all meals, snacks, beverages and condiments provided.

High taurine and saturated fat diet

This is a 3-week (21 day) isocaloric largely plant-based diet with all meals, snacks, beverages and condiments provided.

Low in taurine and saturated fat diet

Rush University Medical Center and University of Illinois at Chicago

Diet Modulation of Bacterial Sulfur and Bile Acid Metabolism and Colon Cancer Risk

Mucosal abundance of bacterial genes associated with sulfur and bile acid metabolism will be measured by quantitative polymerase chain reaction of 16S rRNA and functional genes with biopsy DNA.

Bile salt hydrolase and bile acid 7a-dehydroxylating activities will be measured in stool samples

Measurement of serum bile acids using electrospray-ionization mass spectrometry to indicate the extent of taurine-conjugation of bile acids, ratio of conjugated: unconjugated bile acids and levels of secondary bile acids absorbed from the gut

Gene expression of tumor-necrosis factor-alpha, Interleukin-6, and Cox-2

By COMET fluorescence hybridization assay together with in situ staining for 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), an oxidative DNA lesion, and the expression of its repair enzyme 8-oxoguanine DNA-glycosylase (OGG1; ab91421), XRCC1 [33-2-5] ab1838), which coordinates the action of DNA ligase III, polymerase beta, and poly-ADP-ribose polymerase in the BER pathway and the apurinic/apyrimidinic endonuclease Ape1 (ab2717), a multifunctional protein that protects cells from oxidative stress via its DNA repair, redox, and transcription regulatory activities

Lisa Tussing-Humphreys

Assistant Professor of Medicine

University of Illinois at Chicago

Rush University Medical Center

National Cancer Institute (NCI)

The proposed study will utilize a randomized, crossover, controlled feeding trial design composed of two experimental diets: (1) animal-based, high in taurine and saturated fat (HT-HSAT) and (2) plant-based, low in both taurine and saturated fat (LT-LSAT) (each consumed for 21 days with a minimum 3-week washout between diets).
At baseline and post-diet (day 22) for each of the two 3-week diets, subjects will undergo a flexible sigmoidoscopy, fasting and non-fasting blood draw, and provide a stool sample.

All study personnel, excluding those directly involved with meal preparation, will be blinded to the subjects' diet sequence.

hydrogen sulfide/neoplasms

Diet Modulation of Bacterial Sulfur and Bile Acid Metabolism and Colon Cancer Risk

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