Evidence for type II phytochrome-induced rapid signalling leading to cab::luciferase gene expression in tobacco cotyledons.
Maneno muhimu
Kikemikali
We studied the activation of cab gene expression by phytochrome-induced intercellular signalling and report insights into the mechanism of induction and outspread of a plant internal signal. By micro-beam irradiation techniques and use of a photon-imaging charge-coupled device (CCD) camera system we monitored cab::luciferase reporter gene expression in cotyledons of transgenic tobacco (Nicotiana tabacum L. cv. Xanthi) plants. We found that (i) the photoreceptor triggering intercellular signalling and reporter-gene expression is type II but not type I phytochrome, (ii) phytochrome in its far-red-absorbing (Pfr) form is necessary for the induction but not for the outspread of the signalling, (iii) red/far-red reversibility is restricted to the red-irradiated cells, and (iv) the phytochrome-induced signal spreads rapidly throughout the cotyledon and reaches its target cells within minutes.