Thymus vulgaris Attenuates Myleran-induced Reproductive Damage by Decreasing Oxidative Stress and Lipid Peroxidation in Male Rats

Context: Thymus vulgaris is an herbal with potent antioxidant and it has been shown to have beneficial effects during short-term administration. Myleran (MYL) is used for treatment of certain types of tumors. MYL produces free radicals and induces disturbance in sperm parameters.

Aims: This study is designed to assess the effects of T. vulgaris against damage to the male rats' reproductive features induced by MYL.

Subjects and methods: Sixty-four male Wistar rats were randomly assigned into eight groups: control group; MYL (10 mg/kg) group; T. vulgaris groups (4.5, 9, and 18 mg/kg); and MYL (10 mg/kg) + T. vulgaris groups (4.5, 9, and 18 mg/kg; separately). Treatments were administered daily intraperitoneal injection for 60 days. Total antioxidant capacity, sperm factors, malondialdehyde (MDA), testosterone, and germinal layer height were analyzed.

Results: Whole variables of MYL group decreased signifcantly compared to the control group (P < 0.05) except MDA level (which increased). The T. vulgaris and T. vulgaris + MYL treatments in all doses increased all parameters significantly except MDA level (which decreased) compared to the MYL group (P < 0.05). No significant modifications were observed in all T. vulgaris groups compared to the control group (P > 0.05).

Conclusions: T. vulgaris reduces the poisonous properties of MYL on male reproductive factors.

Keywords: Lipid peroxidation; Myleran; Thymus vulgaris; oxidative stress; reproductive parameters.