A purified hot-water extract from Mycobacterium bovis (BCG vaccine) has been found to have significant antitumor activity against a mouse bladder tumor model and a murine sarcoma in vivo, but not in vitro. The material, termed PS1A1, has an approximate molecular weight of between 60 and 90 kDa, is freely soluble in water, but has low solubility in acetone or ethanol, and is remarkably heat-stable.

Board of Trustees of The University of Illinois

Mixture having antitumor activities

Antibiotic 81-484 of the following physico-chemical properties:(1) elementary analysis: C.sub.33 H.sub.48 O.sub.7 (high resolution mass spectrum);(2) molecular weight: 556 [Field desorption (FD) mass spectrum];(3) melting point: not clear (oily at 10.degree.-100.degree. C.);(4) specific rotation: [.alpha.].sub.D.sup.20 =-151.degree. (c=0.1, methanol);(5) ultraviolet absorption spectrum: shown in FIG. 1 (in methanol);(6) infrared absorption spectrum: shown in FIG. 2 (KBr tablet);(7) solubility: insoluble in hexane and water, soluble in diethyl ether, methanol, ethanol, dichloromethane, chloroform, ethyl acetate, butyl acetate, acetone and benzene;(8) nuclear magnetic resonance spectrum: shown in FIG. 3 (CDCl.sub.3, TMS);(9) nature: acidic substance;(10) color reaction:negative for ninhydrin, anthrone-H.sub.2 SO.sub.4 and ferric chloride reaction;positive for iodine and antimony trichloride;weakly positive for Zatkis reagent;or a non-toxic salt thereof, is produced by culturing Streptomyces 81-484 FERM-P 7371 in a culture medium, and isolating the thus-produced antibiotic therefrom. The antibiotic has no antibiotic activity against Gram-positive and -negative bacteria, has antimicrobial activity against some kinds of fungi, and has growth inhibitory activity against P388 mouse leukemia cells and sarcoma 180 cells.

acetone/sarcoma

Mixture having antitumor activities

Antitumor antibiotic 81-484 and process for its production

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