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Effects of hypoxia on the kinetic and morphological characteristics of human melanoma cells grown as colonies in semi-solid agar medium.

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An investigation was made of the cell populations that occur in the sequential strata of human melanoma ( MM96 ) colonies. Colonies were either grown for the full duration of culture in a 'physiological' atmosphere of 5% O2 (unperturbed colonies), or grown in this atmosphere followed by a final

The role of oxygen limitation in the resistance of agar-entrapped, sessile-like Escherichia coli to aminoglycoside and beta-lactam antibiotics.

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Viable cells of Escherichia coli were entrapped in agar gel layers to form artificial biofilm-like structures. Killing assays of immobilized organisms by latamoxef and tobramycin were performed under different oxygenation conditions of the culture medium and compared with free-cell experiments.

[Over-expression of hypoxia-inducible factor-1alpha induces epithelial-mesenchymal transition in LNCaP cells in vitro and in vivo].

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OBJECTIVE To investigate the effects of the over-expression of hypoxia-inducible factor-1alpha (HIF-1alpha) on the epithelial-mesenchymal transition (EMT) and invasive potency of LNCaP cells in vitro and in vivo. METHODS We cultured LNCaP cells stably expressing HIF-1alpha (LNCaP/HIF-1alpha) and

Transient anoxia during Metarhizium robertsii growth increases conidial virulence to Tenebrio molitor.

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Little is known about the phenotypic effects of hypoxia and transient anoxia on the virulence of an entomopathogenic fungus. Conidia of Metarhizium robertsii were produced on: (1) potato dextrose agar medium (PDA) under normoxia; (2) PDA medium under continuous hypoxia; (3) PDA medium under

Benign-to-malignant B16 melanoma progression induced in two stages in vitro by exposure to hypoxia.

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BACKGROUND Benign tumors apparently become malignant by generating a succession of variants with ever-greater growth potential and autonomy. Such stepwise progression has not been achieved in vitro under conditions likely to occur in developing tumors. OBJECTIVE Tumors initiated by clone G3.5 of the

[Changes in electrical potentials of gastric mucosa during various types of experimental hypoxia].

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In experiments performed on anaesthetized male and female Wistar rats the effects of different types of hypoxia on the electrical potential difference (PD) between the inner and the outer sides of the gastric wall were examined. The PD was determined by mean of the Digital Multimeter VC-10T, Unitra,

[Cancer stem cells play an important role in resistance of laryngeal squamous cancer to irradiation mediated by hypoxia].

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OBJECTIVE To study whether laryngeal cancer stem cells in hypoxia have the characteristic of resistance to irradiation and underlying mechanism. METHODS CD133(+) cells were separated from Hep-2 cells with flow cytometry (FCM) and the purity was 92.8%. The separated CD133(+) cells were cultured in

Mammalian Ste20-like kinase 4 promotes pituitary cell proliferation and survival under hypoxia.

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The genetic and molecular mechanisms that initiate and maintain pituitary tumorigenesis are poorly understood. Nonfunctioning tumors of the gonadotrope lineage represent 35% of all tumors; are usually macroadenomas, often resulting in hypopituitarism; and have no medical treatments. Using expression

Vernolide-A inhibits radiation-induced hypoxia-mediated tumor angiogenesis by regulating HIF-1α, MMP-2, MMP-9, and VEGF.

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We investigated the effect of vernolide-A on the inhibition of radiation-induced tumor angiogenesis in C57BL/6 mice. Vernolide-A administration significantly reduced the tumor volume of radiation-exposed mice. Serum vascular endothelial growth factor (VEGF) levels were drastically elevated during

Distortion product otoacoustic emissions at 6 months in term infants after perinatal hypoxia-ischaemia or with a low Apgar score.

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Distortion product otoacoustic emissions (DPOAEs) were analyzed at 6 months of age in term infants who had perinatal hypoxia-ischaemia (HI) and those who had a low Agar score alone to detect any abnormalities in cochlear function and any difference between the two groups of infants. The f2 primary

[Hypoxia-inducible factor-1α is involved in arsenite-induced epithelial-mesenchymal transition and malignant transformation of human liver epithelial cells via regulating Snail].

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Objective: To investigate the role of hypoxia-inducible factor-1α (HIF-1α) in arsenite-induced epithelial-mesenchymal transition (EMT) and malignant transformation of human liver epithelial cells (L-02 cells). Methods: After the L-02 cells were chronic treated with 2.0 μmol/L NaAsO(2) for 0

A Burrowing/Tunneling Assay for Detection of Hypoxia in Drosophila melanogaster Larvae.

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Oxygen deprivation in animals can result from exposure to low atmospheric oxygen levels or from internal tissue damage that interferes with oxygen distribution. It is also possible that aberrant behavior of oxygen-sensing neurons could induce hypoxia-like behavior in the presence of normal oxygen

Morphologic analysis of three-dimensional tumors developed in fibrin matrix and agar culture system.

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The three-dimensional growth of cultured tumor cell lines (HT29, a colon adenocarcinoma cell line; M21, a melanoma cell line; KB, a nasopharyngeal carcinoma cell line) has been investigated in an agar culture system with a fibrin matrix in vitro. The tumor cells developed to tumors 3 x 3 mm in

Root-zone hypoxia reduces growth of the tropical forage grass Urochloa humidicola in high-nutrient but not low-nutrient conditions.

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The perennial C4 grass Urochloa humidicola is widely planted on infertile acidic and waterlogging-prone soils of tropical America. Waterlogging results in soil anoxia, and O2 deficiency can reduce nutrient uptake by roots. Interestingly, both nutrient deficiencies and soil waterlogging

Gamma linolenic acid suppresses hypoxia-induced proliferation and invasion of non-small cell lung cancer cells by inhibition of HIF1α

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Background: The main therapies for cancer often results in many side effects and drug resistance. Gamma linolenic acid (GLA) is a kind of natural reagent with negligible cytotoxicity. Objective:

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