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Variation in agar growth of transformed 3T3 cells after tumor formation in nude mice.

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Cells from a cloned line of spontaneously transformed 3T3 cells had a colony-forming efficiency in agar (CFEag) of about 10-15% and induced poorly differentiated sarcomas when injected into nude mice. These tumor cells were recultured in vitro and tested for their ability to grow in suspension.

Differentiation of human fibroblasts to tissue macrophages by the Snyder-Theilen feline sarcoma virus (ST:FeSV): growth modulation of human tumor cell lines in agar.

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BACKGROUND Snyder-Theilen feline sarcoma virus (ST:FeSV)-transduced human fibroblasts differentiate into tissue macrophages(1-9). The ST:FeSV-induced macrophages demonstrate macrophage-mediated cytotoxicity (MTC) and antibody-dependent cellular cytotoxicity (ADCC), including growth modulation of

Conversion of human fibroblasts to tissue macrophages by the Snyder-Theilen sarcoma virus (ST:FeSV): tumouricidal effects on K-562 and Daudi tumour cell lines in monolayers and in agar suspensions.

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We have previously demonstrated the conversion of human fibroblasts (HF) to tissue macrophages by transduction with the Snyder-Theilen feline sarcoma virus (ST:FeSV) [1-3]. The ST:FeSV-induced TM have been characterized both phenotypically and functionally, including their tumouricidal potential

Morphometric and colorimetric analyses of human tumor cell line growth and drug sensitivity in soft agar culture.

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Previous studies have demonstrated the suitability of image analysis of tetrazolium-stained colonies to assess growth and drug sensitivity of human tumor cells cultivated in soft agar culture. In the present study, the potential utility of colorimetric analysis to expedite experimental drug

Measuring Survival of Hematopoietic Cancer Cells with the Colony-Forming Assay in Soft Agar.

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Colony-forming assays measure the ability of cells in culture to grow and divide into groups. Any cell that has the potential to form a colony may also have the potential to cause cancer or relapse in vivo. Colony-forming assays also provide an indirect measurement of cell death because any cell

Patterns of tumor colony development over time in soft-agar culture.

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Human tumors were cultured by the two-layer soft-agar technique and the time course of tumor colony development was evaluated during periods of up to 6 weeks in culture. All colony counting was performed with an automated tumor colony counter (Omnicon; Bausch and Lomb, Inc, Rochester, NY, USA). This

In vitro growth characteristics and chemosensitivities of endometrial cancer using a soft agar clonogenic assay.

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A soft agar clonogenic assay was used to analyze 91 fresh human endometrial tumor samples for in vitro growth and chemosensitivities. Overall cloning success was 81%. Of the 75 samples adequate for drug testing (30 or more colonies per plate), histologic analysis demonstrated 36 adenocarcinomas,

Improved optical detection of colony enlargement and drug cytotoxicity in primary soft agar cultures of human solid tumour cells.

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The presence of cellular aggregates in cell suspensions derived from human solid tumours often complicates subsequent evaluation of colony formation in primary soft agar cultures (Agrez et al., 1982b). In the present study, performance of a conventional colony formation assay was observed to lack

Lymphokine-activated killer cells: determination of their tumor cytolytic capacity by a clonogenic microassay using agar capillaries.

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A lymphokine activated killer (LAK) cell assay has been developed using a clonogenic microassay in agar-containing capillaries with KB tumor target cells. The assay avoids the problems of the commonly used 51Cr release assay and mimics physiological conditions more closely. The assay procedure has

Influence of estradiol and tamoxifen on the growth of N-nitrosomethylurea-induced rat mammary tumor cells in soft agar.

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N-Nitrosomethylurea-induced rat mammary tumors were grown in vitro using the clonogenic soft agar technique. Cells from all tumors (n = 46) formed colonies in vitro. Tamoxifen (10(-7), 10(-6) M) inhibited colony formation to 72 and 53% of control values, respectively. The inhibitory effect of

Pharmacologic assessment of regimen chemosensitivity in the soft-agar assay: effect of oxygen on human tumors.

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The influence of oxygen on the growth and the in vitro chemosensitivity of human tumor cells was studied in the soft-agar assay. Tumor cells of pancreatic and ovarian origin prefer a reduced oxygen atmosphere for colony formation, whereas those of pulmonary origin grow better in 20% oxygen.

Soft-agar cultures of transitional cell carcinoma colonies from urine, irrigation fluids and tumor samples.

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Soft-agar cultures of transitional cell carcinoma from urine, irrigation fluid and transurethral resection solid tumor specimens show good colony growth. Growth of tumor colonies produced from urine was adequate to evaluate the presence of a viable tumor, since 12 of 18 noninfected cultures showed

Establishment of a tumor cell line and its use for an agar-free colony-forming assay.

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The establishment of a spontaneously transformed tumorigenic human fibrosarcoma line derived from the ovary of a breast cancer patient (NO-15) is described. This tumor cell line has been characterized by tumor growth in nude mice. The cells form colonies without the usually recommended agar

Endocrine therapy testing of human breast cancers in the soft agar clonogenic assay.

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The human tumor soft agar cloning assay has been used to assess the biological effects of cytotoxic drugs and other agents on human cancers. In this study we have examined the effects of two hormonal agents, tamoxifen (Tam) and medroxyprogesterone acetate (MPA), on colony growth of the MCF-7 human

The proliferation of human tumor cell lines in the presence of different agars, agaroses, and methyl cellulose.

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Human tumor cell lines, derived from cancers of the colon, ovary, and cervix, were grown in liquid tissue culture media and media made semisolid with agar (Bacto + deoxycholate lactose agar), agarose [LE, ME, Sea Plaque and Sea Prep (15/45)], and methyl cellulose. The effects of each agent on

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