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beta-Glucuronidase inhibitor-activity in human saliva and its relation to dental caries, secretor status and blood group.

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[Localization of beta-glucuronidase in human mouth mucosal epithelium].

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The authors investigated the activity of beta-glucuronidase in the mucous membrane of the alveolar process and in the buccal mucous membrane under light and electron microscopes. Demonstration of the enzyme was performed by means of a modification of the method of Hayashi. Under the light microscope

The activity of N-acetyl-β-d-hexosaminidase A and B and β-glucuronidase in nasal polyps and hypertrophic nasal concha.

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Nasal polyps and hypertrophic lower nasal conchae are common disorders of nasal cavity. The majority of etiopathogenetic theories indicate inflammatory background of polyps and hypertrophic concha. N-acetyl-β-D-hexosaminidase and β-glucuronidase are lysosomal exoglycosidases revealing accelerated

Mobilization of leucocytes and subsequent release of histamine and lysosomal enzymes into the peritoneal and pleural cavities of rats by actinomycin D (dactinomycin).

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1. The effects of intraperitoneal injections of actinomycin D on the temporal characteristics of the accumulation of the inflammatory exudate and cells into the peritoneal and pleural cavities were studied in male Sprague Dawley rats. 2. A measurable quantity of the exudate appeared in both cavities

Enzyme activity in the pulp following preparation of cavities and insertion of medicaments in cavities in monkey teeth.

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The effect of cavity preparation, calcium hydroxide and a corticosteroid on pulpal enzymes (Alkaline phosphatase, acid phosphatase, beta-glucuronidase, cytochrome oxidase and succinate, lactate and glucose-6-phosphate dehydrogenase) in monkey teeth has been studied by histochemical means. Cavity

Active site residues of human beta-glucuronidase. Evidence for Glu(540) as the nucleophile and Glu(451) as the acid-base residue.

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Human beta-glucuronidase (hGUSB) is a member of family 2 glycosylhydrolases that cleaves beta-D-glucuronic acid residues from the nonreducing termini of glycosaminoglycans. Amino acid sequence and structural homology of hGUSB and Escherichia coli beta-galactosidase active sites led us to propose

Diagnostic potential of lysosomal hydrolases in body cavity effusions.

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Hexosaminidase, alpha-mannosidase, beta-galactosidase, beta-glucuronidase, and arylsulphatase A were measured inperitoneal and pleural effusions from patients with benign, malignant, and inflammatory disorders. Compared with the benign transudates, all enzyme activities were moderately elevated in

Increased activity of lysosomal enzymes in the peritoneal fluid of bacterial peritonitis.

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OBJECTIVE The activity of lysosomal enzymes is increased in the cerebrospinal fluid of bacterial meningitis, suggesting that inflammation may cause leakage of lysosomal enzymes into the extracellular fluid. Our objective was to study the activity of 3 lysosomal enzymes in cell-free peritoneal fluid

Encapsulation of recombinant cells with a novel magnetized alginate for magnetic resonance imaging.

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Implanting recombinant cells encapsulated in alginate microcapsules to express therapeutic proteins has been proven effective in treating several mouse models of human diseases (neurological disorders, dwarfism, hemophilia, lysosomal storage disease, and cancer). In anticipation of clinical

The effect of Tlr4 and/or C3 deficiency and of neonatal gene therapy on skeletal disease in mucopolysaccharidosis VII mice.

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Mucopolysaccharidosis (MPS) VII is a lysosomal storage disorder caused by the deficiency of the enzyme β-glucuronidase (Gusb(-/-)) and results in glycosaminoglycan (GAG) accumulation. Skeletal abnormalities include stunted long bones and bone degeneration. GAGs have been hypothesized to activate

Glucuronides in mussel Mytilus galloprovincialis as a possible biomonitor of environmental carcinogens.

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1. The in vitro incubation of mussel digestive gland with 1 mM aminofluorene resulted in the formation of glucuronides that (a) became mutagenic with carp liver S9, and (b) liberated S9-dependent mutagenic aglucones after beta-glucuronidase treatment. 2. Natural populations of mussels from

Characterization of hamster tumors induced by simian virus 40 small t deletion mutants as true histiocytic lymphomas.

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Random bred Syrian hamsters given s.c. injections of SV40 small t deletion mutants dl883, dl884, and dl890 rapidly develop reticulum cell sarcomas in the abdominal cavity in addition to slowly developing s.c. fibrosarcomas at the site of virus inoculation. Injection of wild type SV40 s.c. induces

[Protective effect of 764-3 on experimental inflammation].

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The antiinflammatory effect of 764-3 was investigated in rat models of lung inflammation and pleurisy induced by bleomycin intratracheal instillation and carrageenan intrapleural cavity injection respectively. 4 mg/100 g BW.d 764-3 hypodermal injection significantly inhibited the lung inflammatory

Steroids and steroid glucuronides in the ovarian fluid of the African catfish,Clarias gariepinus, between ovulation and oviposition.

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As part of a series of experiments concerning a possible pheromonal function of steroids and steroid glucuronides excreted by the sex organs of the African catfish,Clarias gariepinus, qualitative and quantitative studies, using GCMS, were carried out to examine the presence of the steroids, that can

Observations on the feeding mechanism, diet and digestive physiology of Histriobdella homari Van Beneden 1858: an aberrant polychaete symbiotic with North American and European lobsters.

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1. The aberrant annelid Histriobdella homari (Polychaeta:Eunicida) lives in the branchial chambers of the marine lobsters Homarus americanus and H. vulgaris where it feeds on the rich microflora of bacteria, blue-green algae and related organisms which grow on the inner surface of the branchial

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