The present invention relates to an improved method for collecting blood. The present invention also relates to a method of collecting blood whereby tissue plasminogen, tissue plasminogen activator inhibitor and other serine proteases, serine protease inhibitors and components that are produced or destroyed through the action of serine proteases in collected blood are stabilized. In addition, the present invention provides a method for collecting blood that reduces the hemolysis (lysis of red blood cells). Using the blood collecting method of the present invention, reliable control plasmas can be manufactured.

Biopool International, Inc.

Method of performing tissue plasminogen activator assay

A method for determining the amount of glycated hemoglobin (HbA1c), in which--if required--the erythrocytes in a sample are hemolyzed, the haemoglobin that is then released--if required--is contacted with a proteolytic agent and the glycated hemoglobin degradation products obtained in this way or otherwise are quantified is disclosed. In order to provide such a process and reagents employable therein that has/have the property of sufficient stability of the chemical compounds that are essential to the reaction, the provision of the requisite proteolytic agent in the form of an inactivated protease is proposed, which is then only reactivated in situ. For the stabilization of the hemoglobin, which is unfolded at a very low pH in the range from 1 to 3, at least one suitable stabilizer should be present in the hemolysis solution, and, where a leuco dye is used in connection with the determination of the amount of HbA1c, it is proposed that the latter be stabilized with particular phosphine compounds and/or thio compounds.

DiaSys Diagnostic Systems GmbH

Enzymatic determination of HbA1c

A method for determining the amount of glycated haemoglobin (HbA1c), in which--if required--the erythrocytes in a sample are haemolysed, the haemoglobin that is then released--if required--is contacted with a proteolytic agent and the glycated haemoglobin degradation products obtained in this way or otherwise are quantified is disclosed. In order to provide such a process and reagents employable therein that has/have the property of sufficient stability of the chemical compounds that are essential to the reaction, for the stabilization of the haemoglobin which is unfolded at a very low pH in the range from 1 to 3, at least one suitable stabilizer is present in the haemolysis solution. Where a leuco dye is used in connection with the determination of the amount of HbA1c, it is proposed that the latter be stabilized with particular phosphine compounds and/or thio compounds, and, in particular embodiments, the requisite proteolytic agent is to be provided in the form of an inactivated protease which is then only reactivated in situ.

hemolysis/protease

Method of performing tissue plasminogen activator assay

Enzymatic determination of HbA1c

Enzymatic determination of HBA1c

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