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Lesions of the medial prefrontal cortex enhance the early phase of psychogenic fever to unexpected sucrose concentration reductions, promote recovery from negative contrast and enhance spontaneous recovery of sucrose-entrained anticipatory activity.

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Two groups of rats, one bearing bilateral excitotoxic lesions of the medial prefrontal cortex (mPFC) and one sham-lesioned group, were run in a successive negative contrast paradigm. Both groups had telemeters implanted to monitor core temperature and activity. After ad libitum baseline and food

The use of sucrose-acetone-extracted Rift Valley fever virus antigen derived from cell culture in an indirect enzyme-linked immunosorbent assay and haemagglutination-inhibition test.

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A sucrose-acetone-extracted, Madin-Darby-bovine-kidney (MDBK)-derived Rift Valley fever virus (RVFV) antigen was tested both in an indirect ELISA and a haemagglutination-inhibition test for its ability to detect serum antibodies to RVFV. Optimal conditions for antigen concentration, serum and

Effects of hyperthermia on the sedimentation of nucleoids from HeLa cells in sucrose gradients.

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Failure of hyperthermia to open rat blood-brain barrier: reduced permeation of sucrose.

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Replication of African swine fever virus DNA in infected cells.

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We have examined the ultrastructural localization of African swine fever virus DNA in thin-sections of infected cells by in situ hybridization and autoradiography. Virus-specific DNA sequences were found in the nucleus of infected Vero cells at early times in the synthesis of the viral DNA, forming

Preparation of Rocky Mountain spotted fever vaccine suitable for human immunization.

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Rocky Mountain spotted fever vaccine was produced from rickettsiae grown in chicken embryo cells in roller bottle cultures. The rickettsiae were concentrated and purified by passage through a sucrose gradient and inactivated with formalin. This vaccine satisfactorily passed preinactivation and final

Reactogenicity, immunogenicity, and efficacy of a chick embryo cell-derived vaccine for Rocky Mountain spotted fever.

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A new formalin-inactivated vaccine prepared by sucrose density gradient centrifugation of tissue culture-grown Rickettsia rickettsii was evaluated for safety and immunogenicity in a placebo-controlled, double-blind study. Most of the 52 seronegative adult vaccinees, 88% after the first and 66% after

Sucrose modulation of radiofrequency-induced heating rates and cell death.

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UNASSIGNED Applied radiofrequency (RF) energy induces hyperthermia in tissues, facilitating vascular perfusion This study explores the impact of RF radiation on the integrity of the luminal endothelium, and then predominately explores the impact of altering the conductivity of biologically-relevant

Comparison of the survival on ice of thawed Theileria parva sporozoites of different stocks cryoprotected by glycerol or sucrose.

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Stabilates of Theileria parva sporozoites are mostly delivered in liquid nitrogen tanks to the East Coast fever immunization points. Using an in vitro titration model, we assessed the loss of infectivity of several stabilates when they are stored in ice baths for up to 24 h. Comparisons, with

Hemagglutination-inhibiting antibody in hemorrhagic fever with renal syndrome.

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Hemagglutination activity was demonstrated in a Hantaan virus antigen prepared from infected suckling mouse brain by sucrose-acetone extraction. Hemagglutination of goose erythrocytes was pH dependent and was optimal at pH 6.0-6.2. Immunofluorescent and hemagglutination-inhibiting antibodies were

Gustatory receptor neuron responds to DEET and other insect repellents in the yellow-fever mosquito, Aedes aegypti.

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Three gustatory receptor neurons were characterized for contact chemoreceptive sensilla on the labella of female yellow-fever mosquitoes, Aedes aegypti. The neuron with the smallest amplitude spike responded to the feeding deterrent, quinine, as well as N,N-diethyl-3-methylbenzamide and other insect

Temperature and activity responses to sucrose concentration reductions occur on the 1st but not the 2nd day of concentration shifts, and are blocked by low, constant glucocorticoids.

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Previous findings (N. Pecoraro, J. Chou-Green, & M. F. Dallman, 2003; N. Pecoraro & M. F. Dallman, 2005) indicated that unexpected reductions in sucrose concentration in once daily meals result in a febrile response on the 1st, but not the 2nd day of a concentration shift. This study shows that this

Haemorrhagic fever virus with renal syndrome in small rodents in Czechoslovakia.

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The antigen of haemorrhagic fever with renal syndrome (HFRS) was detected in the lungs of the following free-living small rodents trapped in different localities in Eastern Slovakia: Clethrionomys glareolus (2 positive samples of 7), Apodemus flavicollis (1 sample of 24) and Apodemus agrarius (7

Purification of african malignant catarrhal fever virus using a two-phase aqueous polymer system.

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The WC11 isolate of malignant catarrhal fever virus (MCFV) was purified by a method employing phase separation of the virus in an aqueous polymer system. Virus was grown in primary bovine thyroid cells. The virus released into the extracellular medium was purified. Initial concentration and partial

[Virus-specific proteins of Crimean-Congo hemorrhagic fever].

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A variant of Asian strain Hodzha of Crimean-Congo hemorrhagic fever (Hodzha-A), adapted to Vero-E6 culture and yielding high harvest in it, was used for analysis of the synthesis of virus-specific proteins and protein composition of virions. Major virus-specific proteins with mol. weights 110, 83,

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