Antioxidant drimane-type sesquiterpenoid from muricid gastropod Chicoreus ramosus attenuates pro-inflammatory 5-lipoxygenase and carbolytic enzymes.

The muricid gastropod, Chicoreus ramosus, is a nutrient-enriched food source available along the coastal peninsular of the Indian subcontinent. This study was aimed at bioactivity-directed chromatographic fractionation of the organic extract of C. ramosus to isolate an unprecedented drimane-type sesquiterpenoid Ramosane, characterized as 3-hydroxy-7,9b-dimethyl-5-methylene-8-pentyl-octahydro-1H-cyclopenta[a]naphthalen-9(2H)-one. The compound possessed potential antioxidant activities {2, 2'-diphenyl-1-picryl hydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activities of IC₅₀ 1.42 mM and 1.72 mM, respectively} and was proportionate with those (IC₅₀ 1.39 and 1.69 mM, respectively) exhibited by α-tocopherol. The studied sesquiterpenoid exhibited potential attenuation property countering the pro-inflammatory 5-lipoxygenase (IC₅₀ ~ 4 mM), and its activity was analogous with that exhibited by the anti-inflammatory ibuprofen (IC₅₀ 4.36 mM), whereas its carbolytic α-amylase activity (IC₅₀ 0.96 mM) was commensurate with that displayed by acarbose (IC₅₀ 0.43 mM). The isolated metabolite might anticipate as potential naturally derived bioactive constituent in functional food and pharmaceutical applications. PRACTICAL APPLICATIONS: The edible marine muricid gastropod C. ramosus is a prominently available gastropod species of commercial significance in the coastal regions of Indian Peninsula. An unprecedented drimane-type sesquiterpenoid Ramosane was isolated through the bioactivity-directed chromatographic fractionation of the organic extract of muricidae C. ramosus displaying potential anti-inflammatory and anti-diabetic properties. The present study apprehended the prospective of drimane-type sesquiterpenoid derivative Ramosane purified from C. ramosus as a naturally derived pharmacophore with anti-diabetic and anti-inflammatory potential for utilization in functional food and pharmaceutical formulations to minimize the likelihood of inflammation and hyperglycemic pathologies.