Assessment of the possibility of direct crossing between cultivated potato and two wild allotetraploid relatives.

The cultivated potato Solanum tuberosum L. is an autotetraploid with Endosperm Balance Number (EBN) of 4. There are some allotetraploid 2EBN wild species with desirable traits for potato improvement. However, it is very difficult to cross between these two groups due to differences in EBN. Eleven genotypes of autotetraploid cultivated potato including the six cultivars of subsp. Tuberosum (tbr) and five clones of subsp. Andigena (adg) were crossed with two wild allotetraploid species: S. acaule (acl) and S. stoloniferum (sto). For asses the pollen-pistil incompatibility, some ofpollinated flowers were fixed in Carnoy's solution and stained with aniline blue. Evaluation with fluorescent microscope revealed that pre-zygotic pollen-pistil incompatibility in these crosses is frequent. The pistils of both subsp. of cultivated potato were incompatible with pollens of acl but when sto used as staminate parent it was compatible with five cultivars of subsp. Tuberosum, but incompatible with most ofsubsp. Andigena (4 clones). From 739, 4x, 4EBN x 4x, 2EBN crosses we couldn't get any viable seed. In reciprocal crosses, allotetraploid species were pollinated with a mix of pollens from Tuberosum or Andigena clones. The crosses of sto x adg, acl x adg and acl x tbr were compatible but in the case of sto x tbr the situation was not clear. From these crosses 157 fruits were obtained but most of them contained only shrink seed and we find just one hybrid from the sto x tbr combination. The hybrid was tetraploid (2n = 4x = 48) and vigorous with profuse flowering and good fertility. When this hybrid was used as the pistillate parent it was compatible with both parental species.