Dermatologic evaluation of cosmetic formulations containing Chrysanthemum indicum extract.
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BACKGROUND
Chrysanthemum indicum, an oriental medicinal plant, has been shown to display a variety of pharmacological activities including antibacterial and anti-inflammatory effects.
OBJECTIVE
In this study, we evaluated the ability of C. indicum extracts to inhibit in vitro tyrosinase activity and the skin care effects of cosmetic formulations containing 0.5% C. indicum water extract in human volunteers.
METHODS
The formation of dopachrome from L -dopa by mushroom tyrosinase was observed after treatments with C. indicum extracts. The volunteers received placebo (no extract) or test (0.5% C. indicum water extract) cosmetic cream and applied it on their face three times a day for 6 weeks. Biophysical skin parameters were measured every 2 weeks.
RESULTS
Chrysanthemum indicum methanol and water extracts dose dependently inhibited mushroom tyrosinase activity, and the effects of methanol extract were similar to those of kojic acid, a well-known tyrosinase inhibitor. Clinical evaluations revealed that application of cosmetic formulations containing C. indicum water extract time dependently reduced melanin levels over 6 weeks, whereas the placebo group showed no effect. No changes in moisture, elasticity, wrinkles, evenness, and pore size were observed in either group. HPLC-DAD-ESIMS analyses revealed that luteolin and acacetin-7-O-rutinoside are the major flavonoid compounds in C. indicum water extract.
CONCLUSIONS
These results suggest that C. indicum water extract could be applied as a natural skin-whitening agent for functional cosmetic uses, due to its melanin-reducing efficacy.
