Effects of ethanol extract from Bidens pilosa L. on spontaneous activity, learning and memory in aged rats.

Bidens pilosa L., a herbal medicine, is rich in flavonoids, but its anti-aging effect on neurocognitive functions is not well understood. In the present study, we investigated the effects of ethanol extract from Bidens pilosa L. (EEBP) on spontaneous activity, learning and memory in aged rats.Forty aged (21.90 ± 0.22 months) and 10 young (10 weeks) adult male Sprague-Dawley rats were divided into 5 groups, which were respectively treated orally with 0 mg/kg (young and aged control), 25 mg/kg, 50 mg/kg and 100 mg/kg of EEBP for 30 days consecutively. Then, the animals were examined with open-field, passive avoidance and Morris water maze tasks.In the open-field task, compared with the aged control, the EEBP animals exhibited more rearing (50 mg/kg, P < 0.01) and urination (50 mg/kg, P < 0.01), but less defecation (P < 0.05). In the passive avoidance task, the retention latencies were longer than those in the training phase in all other groups (P < 0.01) except the aged control (P > 0.05). Compared with the young control, the retention latency of the aged control decreased (P < 0.01), but that of the EEBP animals increased again (P < 0.05 vs. aged control). In the Morris water maze, the EEBP animals had shorter latency (100 mg/kg) and had more crossing times (25 mg/kg) in seeking the platform position (P < 0.05, vs. aged control).The results suggested that EEBP could affect the spontaneous activity and improve memory in aged animals and could have potential advantages for cognition improvement in aged populations.