First Report of Sclerotinia sclerotiorum on Lantana camara in Italy.

Lantana camara L. is grown as an ornamental potted plant in Liguria (northern Italy) and is widely used in private and public gardens in central and southern Italy. In some countries, this plant is considered a noxious weed. In the winter of 2007, a sudden wilt was observed on 22-month-old potted plants grown in a commercial greenhouse. Affected plants showed stem necrosis and watery and necrotic leaf tissues. As stem and foliar necrosis progressed, infected plants died. Some plants wilted unilaterally. Infected plants were characterized by the presence of whitish and cottony mycelium. Dark sclerotia were rapidly and abundantly produced on the mycelium. The diseased stem tissue was surface sterilized for 1 min in 1% NaOCl and plated on potato dextrose agar (PDA) amended with 100 mg/l of streptomycin sulfate. Sclerotinia sclerotiorum (Lib.) de Bary (2) was consistently recovered from infected stem pieces. Typical-sized sclerotia were produced on PDA and measured 1.4 to 7.3 × 1.9 to 8.1 (average 3.6 × 4.5) mm. The internal transcribed spacer (ITS) region of rDNA was amplified using the primers ITS4/ITS6 and sequenced. BLAST analysis (1) of the 506-bp segment showed a 100% homology with the sequence of S. sclerotiorum. The nucleotide sequence has been assigned GenBank Accession No. EU 627006. Pathogenicity of two isolates obtained from infected plants was confirmed by inoculating 10 15-month-old plants grown singly in 14-cm-diameter pots. Mycelium plugs, 1 cm², were excised from a 10-day-old PDA culture of each isolate and were placed on the soil surface around the base of each plant. Ten noninoculated plants served as controls. Plants were maintained in a greenhouse under shade at temperatures ranging between 10 and 26°C (average 17°C) and relative humidity >85%. The inoculation trial was carried out twice. All inoculated plants developed leaf yellowing by 10 days after inoculation. White, cottony mycelium and black sclerotia developed on stems and at the base of all inoculated plants. Eventually, the infected plants wilted. Control plants remained symptomless. S. sclerotiorum was reisolated from the stems of inoculated plants. To our knowledge, this is the first report of L. camara as a host of S. sclerotiorum in Italy as well as worldwide. The economic importance of this disease is currently limited. References: (1) S. F. Altschul et al. Nucleic Acids Res. 25:3389, 1997. (2) N. F. Buchwald. Kongl. Veterisk Landb. Aarssk. 75, 1949.