HPLC/UV analysis of polyacetylenes, phenylpropanoid and pyrrolidine alkaloids in medicinally used Codonopsis species.
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BACKGROUND
Codonopsis Radix is commonly used as a tonic in traditional Chinese medicine. However, there is no suitable method to assess the quality of Codonopsis Radix based on multiple components having potential bioactivities.
OBJECTIVE
To establish a HPLC/UV method for simultaneous quantitation of polyacetylenes (lobetyol, lobetyolin, lobetyolinin, cordifolioidyne B), phenylpropanoid (tangshenoside I) and pyrrolidine alkaloids (codonopyrrolidiums A, B) in Codonopsis Radix.
METHODS
Large-scale methanol extraction of Codonopsis Radix, followed by chromatographic separation, provided the seven analytes for quantitation standards. Ultrasound-assisted methanol extracts of samples were analysed using reversed phase, gradient elution HPLC monitored at 215 nm.
RESULTS
The method developed allowed efficient separation of the seven compounds and the detection and quantitation limits of the seven analytes were 0.10-0.32 µg/mL and 0.35-1.07 µg/mL, respectively. All calibration curves showed good linearities (r>0.9993) within the test ranges. Intraday and interday precisions were good with RSD<2.84%. The recoveries of all analytes ranged from 95.8 to 104.7%.
CONCLUSIONS
HPLC/UV is an efficient and accurate method of analysis for simultaneous quantitation of seven components in Codonopsis Radix.
